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Int. J. Mol. Sci. 2016, 17(5), 679; doi:10.3390/ijms17050679

Mechanism of Mitochondrial Connexin43′s Protection of the Neurovascular Unit under Acute Cerebral Ischemia-Reperfusion Injury

Department of Neurology and Neuroscience center, the First Hospital of Jilin University, Changchun 130021, China
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Academic Editor: Katalin Prokai-Tatrai
Received: 4 April 2016 / Revised: 27 April 2016 / Accepted: 29 April 2016 / Published: 5 May 2016
(This article belongs to the Special Issue Neuroprotective Strategies 2016)
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Abstract

We observed mitochondrial connexin43 (mtCx43) expression under cerebral ischemia-reperfusion (I/R) injury, analyzed its regulation, and explored its protective mechanisms. Wistar rats were divided into groups based on injections received before middle cerebral artery occlusion (MCAO). Cerebral infarction volume was detected by 2,3,5-triphenyltetrazolim chloride staining, and cell apoptosis was observed by transferase dUTP nick end labeling. We used transmission electron microscopy to observe mitochondrial morphology and determined superoxide dismutase (SOD) activity and malondialdehyde (MDA) content. MtCx43, p-mtCx43, protein kinase C (PKC), and p-PKC expression were detected by Western blot. Compared with those in the IR group, cerebral infarction volumes in the carbenoxolone (CBX) and diazoxide (DZX) groups were obviously smaller, and the apoptosis indices were down-regulated. Mitochondrial morphology was damaged after I/R, especially in the IR and 5-hydroxydecanoic acid (5-HD) groups. Similarly, decreased SOD activity and increased MDA were observed after MCAO; CBX, DZX, and phorbol-12-myristate-13-acetate (PMA) reduced mitochondrial functional injury. Expression of mtCx43 and p-mtCx43 and the p-Cx43/Cx43 ratio were significantly lower in the IR group than in the sham group. These abnormalities were ameliorated by CBX, DZX, and PMA. MtCx43 may protect the neurovascular unit from acute cerebral IR injury via PKC activation induced by mitoKATP channel agonists. View Full-Text
Keywords: mitochondrial connexin43; mitochondria; cerebral ischemia-reperfusion; mitochondrial ATP-sensitive K+ channel; protein kinase C mitochondrial connexin43; mitochondria; cerebral ischemia-reperfusion; mitochondrial ATP-sensitive K+ channel; protein kinase C
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MDPI and ACS Style

Hou, S.; Shen, P.-P.; Zhao, M.-M.; Liu, X.-P.; Xie, H.-Y.; Deng, F.; Feng, J.-C. Mechanism of Mitochondrial Connexin43′s Protection of the Neurovascular Unit under Acute Cerebral Ischemia-Reperfusion Injury. Int. J. Mol. Sci. 2016, 17, 679.

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