Next Article in Journal
Role of Serum Uric Acid and Ferritin in the Development and Progression of NAFLD
Previous Article in Journal
A Novel Role of Serotonin Receptor 2B Agonist as an Anti-Melanogenesis Agent
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2016, 17(4), 543; doi:10.3390/ijms17040543

The Long Intron 1 of Growth Hormone Gene from Reeves’ Turtle (Chinemys reevesii) Correlates with Negatively Regulated GH Expression in Four Cell Lines

1,2,3,4,†,* , 1,2,3,†
,
1,2,3
,
1,2,3
,
1,2,3
,
1,2,3
,
1,2,3
,
1,2,3
,
1,2,3
,
1,2,3
,
1,2,3
,
1,2,3
,
1,2,3
and
1,2,3,*
1
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, China
2
Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, China
3
Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China
4
College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Stephen Bustin
Received: 29 December 2015 / Revised: 5 April 2016 / Accepted: 6 April 2016 / Published: 12 April 2016
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [2819 KB, uploaded 12 April 2016]   |  

Abstract

Turtles grow slowly and have a long lifespan. Ultrastructural studies of the pituitary gland in Reeves’ turtle (Chinemys reevesii) have revealed that the species possesses a higher nucleoplasmic ratio and fewer secretory granules in growth hormone (GH) cells than other animal species in summer and winter. C. reevesii GH gene was cloned and species-specific similarities and differences were investigated. The full GH gene sequence in C. reevesii contains 8517 base pairs (bp), comprising five exons and four introns. Intron 1 was found to be much longer in C. reevesii than in other species. The coding sequence (CDS) of the turtle’s GH gene, with and without the inclusion of intron 1, was transfected into four cell lines, including DF-1 chicken embryo fibroblasts, Chinese hamster ovary (CHO) cells, human embryonic kidney 293FT cells, and GH4C1 rat pituitary cells; the turtle growth hormone (tGH) gene mRNA and protein expression levels decreased significantly in the intron-containing CDS in these cell lines, compared with that of the corresponding intronless CDS. Thus, the long intron 1 of GH gene in Reeves’ turtle might correlate with downregulated gene expression. View Full-Text
Keywords: turtle; pituitary gland; growth hormone gene; intron; expression turtle; pituitary gland; growth hormone gene; intron; expression
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Liu, W.-S.; Ma, J.-E.; Li, W.-X.; Zhang, J.-G.; Wang, J.; Nie, Q.-H.; Qiu, F.-F.; Fang, M.-X.; Zeng, F.; Wang, X.; Lin, X.-R.; Zhang, L.; Chen, S.-H.; Zhang, X.-Q. The Long Intron 1 of Growth Hormone Gene from Reeves’ Turtle (Chinemys reevesii) Correlates with Negatively Regulated GH Expression in Four Cell Lines. Int. J. Mol. Sci. 2016, 17, 543.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top