Anti-Apoptotic and Pro-Survival Effect of Alpinate Oxyphyllae Fructus (AOF) in a d-Galactose-Induced Aging Heart
AbstractAging, a natural biological/physiological phenomenon, is accelerated by reactive oxygen species (ROS) accumulation and identified by a progressive decrease in physiological function. Several studies have shown a positive relationship between aging and chronic heart failure (HF). Cardiac apoptosis was found in age-related diseases. We used a traditional Chinese medicine, Alpinate Oxyphyllae Fructus (AOF), to evaluate its effect on cardiac anti-apoptosis and pro-survival. Male eight-week-old Sprague–Dawley (SD) rats were segregated into five groups: normal control group (NC), d-Galactose-Induced aging group (Aging), and AOF of 50 (AL (AOF low)), 100 (AM (AOF medium)), 150 (AH (AOF high)) mg/kg/day. After eight weeks, hearts were measured by an Hematoxylin–Eosin (H&E) stain, Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-assays and Western blotting. The experimental results show that the cardiomyocyte apoptotic pathway protein expression increased in the d-Galactose-Induced aging groups, with dose-dependent inhibition in the AOF treatment group (AL, AM, and AH). Moreover, the expression of the pro-survival p-Akt (protein kinase B (Akt)), Bcl-2 (B-cell lymphoma 2), anti-apoptotic protein (Bcl-xL) protein decreased significantly in the d-Galactose-induced aging group, with increased performance in the AOF treatment group with levels of p-IGFIR and p-PI3K (Phosphatidylinositol-3′ kinase (PI3K)) to increase by dosage and compensatory performance. On the other hand, the protein of the Sirtuin 1 (SIRT1) pathway expression decreased in the aging groups and showed improvement in the AOF treatment group. Our results suggest that AOF strongly works against ROS-induced aging heart problems. View Full-Text
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Chang, Y.-M.; Chang, H.-H.; Kuo, W.-W.; Lin, H.-J.; Yeh, Y.-L.; Padma Viswanadha, V.; Tsai, C.-C.; Chen, R.-J.; Chang, H.-N.; Huang, C.-Y. Anti-Apoptotic and Pro-Survival Effect of Alpinate Oxyphyllae Fructus (AOF) in a d-Galactose-Induced Aging Heart. Int. J. Mol. Sci. 2016, 17, 466.
Chang Y-M, Chang H-H, Kuo W-W, Lin H-J, Yeh Y-L, Padma Viswanadha V, Tsai C-C, Chen R-J, Chang H-N, Huang C-Y. Anti-Apoptotic and Pro-Survival Effect of Alpinate Oxyphyllae Fructus (AOF) in a d-Galactose-Induced Aging Heart. International Journal of Molecular Sciences. 2016; 17(4):466.Chicago/Turabian Style
Chang, Yung-Ming; Chang, Hen-Hong; Kuo, Wei-Wen; Lin, Hung-Jen; Yeh, Yu-Lan; Padma Viswanadha, Vijaya; Tsai, Chin-Chuan; Chen, Ray-Jade; Chang, Hsin-Nung; Huang, Chih-Yang. 2016. "Anti-Apoptotic and Pro-Survival Effect of Alpinate Oxyphyllae Fructus (AOF) in a d-Galactose-Induced Aging Heart." Int. J. Mol. Sci. 17, no. 4: 466.
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