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Int. J. Mol. Sci. 2016, 17(4), 466; doi:10.3390/ijms17040466

Anti-Apoptotic and Pro-Survival Effect of Alpinate Oxyphyllae Fructus (AOF) in a d-Galactose-Induced Aging Heart

The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, Kaohsiung 84001, Taiwan
Chinese Medicine Department, E-DA Hospital, Kaohsiung 82445, Taiwan
1PT Biotechnology Co., Ltd., Taichung 433, Taiwan
Research Center for Chinese Medicine & Acupuncture, China Medical University, Taichung 40402, Taiwan
Departments of Chinese Medicine, China Medical University Hospital, Taichung 40447, Taiwan
School of Post-Baccalaureate Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan
Department of Biological Science and Technology, China Medical University, Taichung 40447, Taiwan
Department of pathology, Changhua Christian Hospital, Changhua 50506, Taiwan
Department of Medical Technology, Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli 35665, Taiwan
Department of Biotechnology, Bharathiar University, Coimbatore 641046, India
Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11042, Taiwan
Graduate Institute of Basic Medical Science, China Medical University, Taichung 40402, Taiwan
School of Chinese Medicine, China Medical University, Taichung 40447, Taiwan
Department of Health and Nutrition Biotechnology, Asia University, Taichung 41354, Taiwan
Author to whom correspondence should be addressed.
Academic Editor: Harry A. J. Struijker-Boudier
Received: 27 January 2016 / Revised: 21 March 2016 / Accepted: 21 March 2016 / Published: 29 March 2016
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [3903 KB, uploaded 29 March 2016]   |  


Aging, a natural biological/physiological phenomenon, is accelerated by reactive oxygen species (ROS) accumulation and identified by a progressive decrease in physiological function. Several studies have shown a positive relationship between aging and chronic heart failure (HF). Cardiac apoptosis was found in age-related diseases. We used a traditional Chinese medicine, Alpinate Oxyphyllae Fructus (AOF), to evaluate its effect on cardiac anti-apoptosis and pro-survival. Male eight-week-old Sprague–Dawley (SD) rats were segregated into five groups: normal control group (NC), d-Galactose-Induced aging group (Aging), and AOF of 50 (AL (AOF low)), 100 (AM (AOF medium)), 150 (AH (AOF high)) mg/kg/day. After eight weeks, hearts were measured by an Hematoxylin–Eosin (H&E) stain, Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-assays and Western blotting. The experimental results show that the cardiomyocyte apoptotic pathway protein expression increased in the d-Galactose-Induced aging groups, with dose-dependent inhibition in the AOF treatment group (AL, AM, and AH). Moreover, the expression of the pro-survival p-Akt (protein kinase B (Akt)), Bcl-2 (B-cell lymphoma 2), anti-apoptotic protein (Bcl-xL) protein decreased significantly in the d-Galactose-induced aging group, with increased performance in the AOF treatment group with levels of p-IGFIR and p-PI3K (Phosphatidylinositol-3′ kinase (PI3K)) to increase by dosage and compensatory performance. On the other hand, the protein of the Sirtuin 1 (SIRT1) pathway expression decreased in the aging groups and showed improvement in the AOF treatment group. Our results suggest that AOF strongly works against ROS-induced aging heart problems. View Full-Text
Keywords: AOF; d-galactose-induced aging; apoptosis; SIRT1 AOF; d-galactose-induced aging; apoptosis; SIRT1

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Chang, Y.-M.; Chang, H.-H.; Kuo, W.-W.; Lin, H.-J.; Yeh, Y.-L.; Padma Viswanadha, V.; Tsai, C.-C.; Chen, R.-J.; Chang, H.-N.; Huang, C.-Y. Anti-Apoptotic and Pro-Survival Effect of Alpinate Oxyphyllae Fructus (AOF) in a d-Galactose-Induced Aging Heart. Int. J. Mol. Sci. 2016, 17, 466.

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