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Int. J. Mol. Sci. 2016, 17(4), 410; doi:10.3390/ijms17040410

Identification of Serum Peptidome Signatures of Non-Small Cell Lung Cancer

1
Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Grunwaldzka 6 Street, 60-780 Poznan, Poland
2
Department of Thoracic Surgery, Poznan University of Medical Sciences, Szamarzewskiego 62 Street, 60-569 Poznan, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: William Chi-shing Cho
Received: 13 January 2016 / Revised: 7 March 2016 / Accepted: 14 March 2016 / Published: 31 March 2016
(This article belongs to the Special Issue Molecular Classification of Human Cancer: Diagnosis and Treatment)
View Full-Text   |   Download PDF [431 KB, uploaded 31 March 2016]   |  

Abstract

Due to high mortality rates of lung cancer, there is a need for identification of new, clinically useful markers, which improve detection of this tumor in early stage of disease. In the current study, serum peptide profiling was evaluated as a diagnostic tool for non-small cell lung cancer patients. The combination of the ZipTip technology with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for the analysis of peptide pattern of cancer patients (n = 153) and control subjects (n = 63) was presented for the first time. Based on the observed significant differences between cancer patients and control subjects, the classification model was created, which allowed for accurate group discrimination. The model turned out to be robust enough to discriminate a new validation set of samples with satisfactory sensitivity and specificity. Two peptides from the diagnostic pattern for non-small cell lung cancer (NSCLC) were identified as fragments of C3 and fibrinogen α chain. Since ELISA test did not confirm significant differences in the expression of complement component C3, further study will involve a quantitative approach to prove clinical utility of the other proteins from the proposed multi-peptide cancer signature. View Full-Text
Keywords: peptide profiling; non-small cell lung cancer; MALDI-TOF-MS; ZipTip enrichment peptide profiling; non-small cell lung cancer; MALDI-TOF-MS; ZipTip enrichment
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Klupczynska, A.; Swiatly, A.; Hajduk, J.; Matysiak, J.; Dyszkiewicz, W.; Pawlak, K.; Kokot, Z.J. Identification of Serum Peptidome Signatures of Non-Small Cell Lung Cancer. Int. J. Mol. Sci. 2016, 17, 410.

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