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Int. J. Mol. Sci. 2016, 17(3), 269; doi:10.3390/ijms17030269

Nutritional Signaling Regulates Vitellogenin Synthesis and Egg Development through Juvenile Hormone in Nilaparvata lugens (Stål)

1
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
*
Author to whom correspondence should be addressed.
Academic Editor: Lee A. Bulla
Received: 22 December 2015 / Revised: 2 February 2016 / Accepted: 14 February 2016 / Published: 26 February 2016
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [2237 KB, uploaded 26 February 2016]   |  

Abstract

Insect female reproduction which comprises the synthesis of vitellogenein (Vg) in the fat body and its incorporation into developing oocytes, needs a large amount of energy and food resources. Our previous studies found that juvenile hormone (JH) regulates vitellogenesis in the brown planthopper, Nilaparvata lugens. Here, we report on the role of JH in nutrient-regulated Vg synthesis and egg development. We first cloned the genes coding for juvenile hormone acid methyltransferase (JHAMT) which is involved in JH biosynthesis and methoprene-tolerant (Met) for JH action. Amino acids (AAs) induced the expression of jmtN, while showing no effects on the expression of met using an artificial diet culture system. Reduction in JH biosynthesis or its action by RNA interference (RNAi)-mediated silencing of jmtN or met led to a severe inhibition of AAs-induced Vg synthesis and oocyte maturation, together with lower fecundity. Furthermore, exogenous application of JH III partially restored Vg expression levels in jmtN RNAi females. However, JH III application did not rescue Vg synthesis in these met RNAi insects. Our results show that AAs induce Vg synthesis in the fat body and egg development in concert with JH biosynthesis in Nilaparvata lugens (Stål), rather than through JH action. View Full-Text
Keywords: Juvenile hormone; vitellogenesis; nutritional signaling; RNA inteference; Nilaparvata lugens Juvenile hormone; vitellogenesis; nutritional signaling; RNA inteference; Nilaparvata lugens
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MDPI and ACS Style

Lu, K.; Chen, X.; Liu, W.-T.; Zhang, X.-Y.; Chen, M.-X.; Zhou, Q. Nutritional Signaling Regulates Vitellogenin Synthesis and Egg Development through Juvenile Hormone in Nilaparvata lugens (Stål). Int. J. Mol. Sci. 2016, 17, 269.

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