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Int. J. Mol. Sci. 2016, 17(2), 251; doi:10.3390/ijms17020251

Metabolic Activity of Radish Sprouts Derived Isothiocyanates in Drosophila melanogaster

1
Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Strasse 6, 24118 Kiel, Germany
2
Department of Food Science and Technology, CEBAS-CSIC, Campus de Espinardo 25, 30100 Murcia, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Manickam Sugumaran
Received: 13 January 2016 / Revised: 4 February 2016 / Accepted: 5 February 2016 / Published: 18 February 2016
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

We used Drosophila melanogaster as a model system to study the absorption, metabolism and potential health benefits of plant bioactives derived from radish sprouts (Raphanus sativus cv. Rambo), a Brassicaceae species rich in glucosinolates and other phytochemicals. Flies were subjected to a diet supplemented with lyophilized radish sprouts (10.6 g/L) for 10 days, containing high amounts of glucoraphenin and glucoraphasatin, which can be hydrolyzed by myrosinase to the isothiocyanates sulforaphene and raphasatin, respectively. We demonstrate that Drosophila melanogaster takes up and metabolizes isothiocyanates from radish sprouts through the detection of the metabolite sulforaphane-cysteine in fly homogenates. Moreover, we report a decrease in the glucose content of flies, an upregulation of spargel expression, the Drosophila homolog of the mammalian PPARγ-coactivator 1 α, as well as the inhibition of α-amylase and α-glucosidase in vitro. Overall, we show that the consumption of radish sprouts affects energy metabolism in Drosophila melanogaster which is reflected by lower glucose levels and an increased expression of spargel, a central player in mitochondrial biogenesis. These processes are often affected in chronic diseases associated with aging, including type II diabetes mellitus. View Full-Text
Keywords: Brassicaceae; sulforaphene; radish; spargel; energy metabolism Brassicaceae; sulforaphene; radish; spargel; energy metabolism
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Baenas, N.; Piegholdt, S.; Schloesser, A.; Moreno, D.A.; García-Viguera, C.; Rimbach, G.; Wagner, A.E. Metabolic Activity of Radish Sprouts Derived Isothiocyanates in Drosophila melanogaster. Int. J. Mol. Sci. 2016, 17, 251.

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