A Classic Near-Infrared Probe Indocyanine Green for Detecting Singlet Oxygen
AbstractThe revelation of mechanisms of photodynamic therapy (PDT) at the cellular level as well as singlet oxygen (1O2) as a second messengers requires the quantification of intracellular 1O2. To detect singlet oxygen, directly measuring the phosphorescence emitted from 1O2 at 1270 nm is simple but limited for the low quantum yield and intrinsic efficiency of 1O2 emission. Another method is chemically trapping 1O2 and measuring fluorescence, absorption and Electron Spin Resonance (ESR). In this paper, we used indocyanine green (ICG), the only near-infrared (NIR) probe approved by the Food and Drug Administration (FDA), to detect 1O2 in vitro. Once it reacts with 1O2, ICG is decomposed and its UV absorption at 780 nm decreases with the laser irradiation. Our data demonstrated that ICG could be more sensitive and accurate than Singlet Oxygen Sensor Green reagent® (SOSG, a commercialized fluorescence probe) in vitro, moreover, ICG functioned with Eosin Y while SOSG failed. Thus, ICG would reasonably provide the possibility to sense 1O2 in vitro, with high sensitivity, selectivity and suitability to most photosensitizers. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 1241 KB)
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Tang, C.-Y.; Wu, F.-Y.; Yang, M.-K.; Guo, Y.-M.; Lu, G.-H.; Yang, Y.-H. A Classic Near-Infrared Probe Indocyanine Green for Detecting Singlet Oxygen. Int. J. Mol. Sci. 2016, 17, 219.
Tang C-Y, Wu F-Y, Yang M-K, Guo Y-M, Lu G-H, Yang Y-H. A Classic Near-Infrared Probe Indocyanine Green for Detecting Singlet Oxygen. International Journal of Molecular Sciences. 2016; 17(2):219.Chicago/Turabian Style
Tang, Cheng-Yi; Wu, Feng-Yao; Yang, Min-Kai; Guo, Yu-Min; Lu, Gui-Hua; Yang, Yong-Hua. 2016. "A Classic Near-Infrared Probe Indocyanine Green for Detecting Singlet Oxygen." Int. J. Mol. Sci. 17, no. 2: 219.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.