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Int. J. Mol. Sci. 2016, 17(12), 2110; doi:10.3390/ijms17122110

Validating Intravascular Imaging with Serial Optical Coherence Tomography and Confocal Fluorescence Microscopy

1
Département de Génie Électrique et Institut de Génie Biomédical, École Polytechnique de Montréal, Montreal, QC H3T 1J4, Canada
2
Montreal Heart Institute, Montreal, QC H1T 1C8, Canada
3
Département de Médecine, Université de Montréal, Montreal, QC H3C 3J7, Canada
4
National Research Council Canada (NRCC), Montreal, QC H3A 1A3, Canada
*
Authors to whom correspondence should be addressed.
Academic Editors: Michael Henein and Joseph V. Moxon
Received: 31 August 2016 / Revised: 25 November 2016 / Accepted: 9 December 2016 / Published: 15 December 2016
(This article belongs to the Special Issue Atherosclerosis and Vascular Imaging 2016)
View Full-Text   |   Download PDF [7720 KB, uploaded 15 December 2016]   |  

Abstract

Atherosclerotic cardiovascular diseases are characterized by the formation of a plaque in the arterial wall. Intravascular ultrasound (IVUS) provides high-resolution images allowing delineation of atherosclerotic plaques. When combined with near infrared fluorescence (NIRF), the plaque can also be studied at a molecular level with a large variety of biomarkers. In this work, we present a system enabling automated volumetric histology imaging of excised aortas that can spatially correlate results with combined IVUS/NIRF imaging of lipid-rich atheroma in cholesterol-fed rabbits. Pullbacks in the rabbit aortas were performed with a dual modality IVUS/NIRF catheter developed by our group. Ex vivo three-dimensional (3D) histology was performed combining optical coherence tomography (OCT) and confocal fluorescence microscopy, providing high-resolution anatomical and molecular information, respectively, to validate in vivo findings. The microscope was combined with a serial slicer allowing for the imaging of the whole vessel automatically. Colocalization of in vivo and ex vivo results is demonstrated. Slices can then be recovered to be tested in conventional histology. View Full-Text
Keywords: intravascular ultrasound (IVUS); near-infrared fluorescence (NIRF); atherosclerosis; ex vivo three-dimensional (3D) histology; optical coherence tomography (OCT); confocal fluorescence microscopy intravascular ultrasound (IVUS); near-infrared fluorescence (NIRF); atherosclerosis; ex vivo three-dimensional (3D) histology; optical coherence tomography (OCT); confocal fluorescence microscopy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Tardif, P.-L.; Bertrand, M.-J.; Abran, M.; Castonguay, A.; Lefebvre, J.; Stähli, B.E.; Merlet, N.; Mihalache-Avram, T.; Geoffroy, P.; Mecteau, M.; Busseuil, D.; Ni, F.; Abulrob, A.; Rhéaume, É.; L’Allier, P.; Tardif, J.-C.; Lesage, F. Validating Intravascular Imaging with Serial Optical Coherence Tomography and Confocal Fluorescence Microscopy. Int. J. Mol. Sci. 2016, 17, 2110.

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