Next Article in Journal
Testosterone Deficiency Induces Changes of the Transcriptomes of Visceral Adipose Tissue in Miniature Pigs Fed a High-Fat and High-Cholesterol Diet
Next Article in Special Issue
Identification and Characterization of Lipopolysaccharide Induced TNFα Factor from Blunt Snout Bream, Megalobrama amblycephala
Previous Article in Journal
Microbial Etiology of Pneumonia: Epidemiology, Diagnosis and Resistance Patterns
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2016, 17(12), 1921; doi:10.3390/ijms17121921

Aspirin down Regulates Hepcidin by Inhibiting NF-κB and IL6/JAK2/STAT3 Pathways in BV-2 Microglial Cells Treated with Lipopolysaccharide

1,2,†
,
1,†
,
1
,
2,* , 1,3
,
3,* and 1,*
1
Laboratory of Neuropharmacology, Fudan University School of Pharmacy, Shanghai 201203, China
2
Department of Neurology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China
3
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Juan M. Tomás
Received: 30 September 2016 / Revised: 26 October 2016 / Accepted: 9 November 2016 / Published: 16 December 2016
(This article belongs to the Special Issue Lipopolysaccharides (LPSs))
View Full-Text   |   Download PDF [1455 KB, uploaded 16 December 2016]   |  

Abstract

Aspirin down regulates transferrin receptor 1 (TfR1) and up regulates ferroportin 1 (Fpn1) and ferritin expression in BV-2 microglial cells treated without lipopolysaccharides (LPS), as well as down regulates hepcidin and interleukin 6 (IL-6) in cells treated with LPS. However, the relevant mechanisms are unknown. Here, we investigate the effects of aspirin on expression of hepcidin and iron regulatory protein 1 (IRP1), phosphorylation of Janus kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3) and P65 (nuclear factor-κB), and the production of nitric oxide (NO) in BV-2 microglial cells treated with and without LPS. We demonstrated that aspirin inhibited hepcidin mRNA as well as NO production in cells treated with LPS, but not in cells without LPS, suppresses IL-6, JAK2, STAT3, and P65 (nuclear factor-κB) phosphorylation and has no effect on IRP1 in cells treated with or without LPS. These findings provide evidence that aspirin down regulates hepcidin by inhibiting IL6/JAK2/STAT3 and P65 (nuclear factor-κB) pathways in the cells under inflammatory conditions, and imply that an aspirin-induced reduction in TfR1 and an increase in ferritin are not associated with IRP1 and NO. View Full-Text
Keywords: aspirin; hepcidin; P65 (nuclear factor-κB); IL-6/JAK2/STAT3 pathway; lipopolysaccharide (LPS); nitric oxide (NO); iron regulatory protein 1 (IRP1) aspirin; hepcidin; P65 (nuclear factor-κB); IL-6/JAK2/STAT3 pathway; lipopolysaccharide (LPS); nitric oxide (NO); iron regulatory protein 1 (IRP1)
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Li, W.-Y.; Li, F.-M.; Zhou, Y.-F.; Wen, Z.-M.; Ma, J.; Ya, K.; Qian, Z.-M. Aspirin down Regulates Hepcidin by Inhibiting NF-κB and IL6/JAK2/STAT3 Pathways in BV-2 Microglial Cells Treated with Lipopolysaccharide. Int. J. Mol. Sci. 2016, 17, 1921.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top