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Int. J. Mol. Sci. 2017, 18(2), 233; doi:10.3390/ijms18020233

Identification and Characterization of Lipopolysaccharide Induced TNFα Factor from Blunt Snout Bream, Megalobrama amblycephala

1
Department of Cell Biology, School of Life Sciences, Central South University, Changsha 410017, China
2
Center of Biological Experiments, School of Life Sciences, Central South University, Changsha 410017, China
3
Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning 530021, China
*
Author to whom correspondence should be addressed.
Academic Editor: Juan M. Tomás
Received: 19 October 2016 / Revised: 17 January 2017 / Accepted: 18 January 2017 / Published: 15 February 2017
(This article belongs to the Special Issue Lipopolysaccharides (LPSs))
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Abstract

Lipopolysaccharide induced TNFα factor (LITAF) is an important transcription factor responsible for regulation of tumor necrosis factor α. In this study, a novel litaf gene (designated as Malitaf) was identified and characterized from blunt snout bream, Megalobrama amblycephala. The full-length cDNA of Malitaf was of 956 bp, encoding a polypeptide of 161 amino acids with high similarity to other known LITAFs. A phylogenetic tree also showed that Malitaf significantly clustered with those of other teleost, indicating that Malitaf was a new member of fish LITAF family. The putative maLITAF protein possessed a highly conserved LITAF domain with two CXXC motifs. The mRNA transcripts of Malitaf were detected in all examined tissues of healthy M. amblycephala, including kidney, head kidney, muscle, liver, spleen, gill, and heart, and with the highest expression in immune organs: spleen and head kidney. The expression level of Malitaf in spleen was rapidly up-regulated and peaked (1.29-fold, p < 0.05) at 2 h after lipopolysaccharide (LPS) stimulation. Followed the stimulation of Malitaf, Matnfα transcriptional level was also transiently induced to a high level (51.74-fold, p < 0.001) at 4 h after LPS stimulation. Taken together, we have identified a putative fish LITAF ortholog, which was a constitutive and inducible immune response gene involved in M. amblycephala innate immunity during the course of a pathogenic infection. View Full-Text
Keywords: Megalobrama amblycephala; lipopolysaccharide induced TNFα factor; lipopolysaccharide stimulation; innate immune Megalobrama amblycephala; lipopolysaccharide induced TNFα factor; lipopolysaccharide stimulation; innate immune
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MDPI and ACS Style

Lv, Y.; Xiang, X.; Jiang, Y.; Tang, L.; Zhou, Y.; Zhong, H.; Xiao, J.; Yan, J. Identification and Characterization of Lipopolysaccharide Induced TNFα Factor from Blunt Snout Bream, Megalobrama amblycephala. Int. J. Mol. Sci. 2017, 18, 233.

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