Validated HPAEC-PAD Method for the Determination of Fully Deacetylated Chitooligosaccharides
AbstractAn efficient and sensitive analytical method based on high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was established for the simultaneous separation and determination of glucosamine (GlcN)1 and chitooligosaccharides (COS) ranging from (GlcN)2 to (GlcN)6 without prior derivatization. Detection limits were 0.003 to 0.016 mg/L (corresponding to 0.4–0.6 pmol), and the linear range was 0.2 to 10 mg/L. The optimized analysis was carried out on a CarboPac-PA100 analytical column (4 × 250 mm) using isocratic elution with 0.2 M aqueous sodium hydroxide-water mixture (10:90, v/v) as the mobile phase at a 0.4 mL/min flow rate. Regression equations revealed a good linear relationship (R2 = 0.9979–0.9995, n = 7) within the test ranges. Quality parameters, including precision and accuracy, were fully validated and found to be satisfactory. The fully validated HPAEC-PAD method was readily applied for the quantification of (GlcN)1–6 in a commercial COS technical concentrate. The established method was also used to monitor the acid hydrolysis of a COS technical concentrate to ensure optimization of reaction conditions and minimization of (GlcN)1 degradation. View Full-Text
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Cao, L.; Wu, J.; Li, X.; Zheng, L.; Wu, M.; Liu, P.; Huang, Q. Validated HPAEC-PAD Method for the Determination of Fully Deacetylated Chitooligosaccharides. Int. J. Mol. Sci. 2016, 17, 1699.
Cao L, Wu J, Li X, Zheng L, Wu M, Liu P, Huang Q. Validated HPAEC-PAD Method for the Determination of Fully Deacetylated Chitooligosaccharides. International Journal of Molecular Sciences. 2016; 17(10):1699.Chicago/Turabian Style
Cao, Lidong; Wu, Jinlong; Li, Xiuhuan; Zheng, Li; Wu, Miaomiao; Liu, Pingping; Huang, Qiliang. 2016. "Validated HPAEC-PAD Method for the Determination of Fully Deacetylated Chitooligosaccharides." Int. J. Mol. Sci. 17, no. 10: 1699.
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