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Int. J. Mol. Sci. 2016, 17(10), 1577; doi:10.3390/ijms17101577

Characterization of a β-Adrenergic-Like Octopamine Receptor in the Oriental Fruit Fly, Bactrocera dorsalis (Hendel)

1
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China
2
Department of Crop Protection, Ghent University, Ghent 9000, Belgium
*
Author to whom correspondence should be addressed.
Academic Editor: T. G. Emyr Davies
Received: 29 June 2016 / Revised: 4 September 2016 / Accepted: 13 September 2016 / Published: 22 September 2016
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

The biogenic amine octopamine plays a critical role in the regulation of many physiological processes in insects. Octopamine transmits its action through a set of specific G-protein coupled receptors (GPCRs), namely octopamine receptors. Here, we report on a β-adrenergic-like octopamine receptor gene (BdOctβR1) from the oriental fruit fly, Bactrocera dorsalis (Hendel), a destructive agricultural pest that occurs in North America and the Asia-Pacific region. As indicated by RT-qPCR, BdOctβR1 was highly expressed in the central nervous system (CNS) and Malpighian tubules (MT) in the adult flies, suggesting it may undertake important roles in neural signaling in the CNS as well as physiological functions in the MT of this fly. Furthermore, its ligand specificities were tested in a heterologous expression system where BdOctβR1 was expressed in HEK-293 cells. Based on cyclic AMP response assays, we found that BdOctβR1 could be activated by octopamine in a concentration-dependent manner, confirming that this receptor was functional, while tyramine and dopamine had much less potency than octopamine. Naphazoline possessed the highest agonistic activity among the tested agonists. In antagonistic assays, mianserin had the strongest activity and was followed by phentolamine and chlorpromazine. Furthermore, when the flies were kept under starvation, there was a corresponding increase in the transcript level of BdOctβR1, while high or low temperature stress could not induce significant expression changes. The above results suggest that BdOctβR1 may be involved in the regulation of feeding processes in Bactrocera dorsalis and may provide new potential insecticide leads targeting octopamine receptors. View Full-Text
Keywords: biogenic amine; Bactrocera dorsalis; octopamine receptor; cyclic AMP; agonist; antagonist; functional expression; stress biogenic amine; Bactrocera dorsalis; octopamine receptor; cyclic AMP; agonist; antagonist; functional expression; stress
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Li, H.-M.; Jiang, H.-B.; Gui, S.-H.; Liu, X.-Q.; Liu, H.; Lu, X.-P.; Smagghe, G.; Wang, J.-J. Characterization of a β-Adrenergic-Like Octopamine Receptor in the Oriental Fruit Fly, Bactrocera dorsalis (Hendel). Int. J. Mol. Sci. 2016, 17, 1577.

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