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Int. J. Mol. Sci. 2016, 17(1), 72; doi:10.3390/ijms17010072

MiRNA-Target Interaction Reveals Cell-Specific Post-Transcriptional Regulation in Mammalian Cell Lines

Department of Periodontics, College of Dentistry, University of Illinois at Chicago, 458 Dent MC 859, 801 S. Paulina, Chicago, IL 60612, USA
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Academic Editor: Constantinos Stathopoulos
Received: 20 November 2015 / Revised: 21 December 2015 / Accepted: 31 December 2015 / Published: 8 January 2016
(This article belongs to the Collection Regulation by Non-Coding RNAs)
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Abstract

MicroRNAs are 18–22 nucleotides long, non-coding RNAs that bind transcripts with complementary sequences leading to either mRNA degradation or translational suppression. However, the inherent differences in preferred mode of miRNA regulation among cells of different origin have not been examined. In our previous transcriptome profiling studies, we observed that post-transcriptional regulation can differ substantially depending on the cell in context. Here we examined mechanistic differences in the regulation of a let-7a targeted (wild type) or resistant (mutant) engineered renilla transcript across various mammalian cell lines of diverse origin. Dual luciferase assays show that compared to mutant (mut), the reporter gene containing wild type (wt) let-7a binding sites was efficiently suppressed upon transfection in various cell lines. Importantly, the strength of miRNA regulation varied across the cell lines. Total RNA analysis demonstrates that wt renilla mRNA was expressed to similar or higher levels compared to mut suggesting that translation repression is a predominant mode of miRNA regulation. Nonetheless, transcript degradation was observed in some cell lines. Ago-2 immunoprecipitation show that miRNA repressed renilla mRNA are associated with functional mi-RISC (miRNA-RNA induced silencing complex). Given the immense potential of miRNA as a therapeutic option, these findings highlight the necessity to thoroughly examine the mode of mRNA regulation in order to achieve the beneficial effects in targeting cells. View Full-Text
Keywords: microRNA; gene regulation; cell lines; Ago-2 (Argonaute-2) microRNA; gene regulation; cell lines; Ago-2 (Argonaute-2)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kulkarni, V.; Naqvi, A.R.; Uttamani, J.R.; Nares, S. MiRNA-Target Interaction Reveals Cell-Specific Post-Transcriptional Regulation in Mammalian Cell Lines. Int. J. Mol. Sci. 2016, 17, 72.

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