Stable Expression of Basic Fibroblast Growth Factor in Chloroplasts of Tobacco
AbstractBasic fibroblast growth factor (bFGF) is a multifunctional factor in acceleration of cell proliferation, differentiation and transference, and therefore widely used in clinical applications. In this study, expression vector pWX-Nt03 harboring a codon-optimized bFGF gene was constructed and introduced into the tobacco chloroplasts by particle bombardment. After four rounds of selection, bFGF was proved to integrate into the chloroplast genome of regenerated plants and two of four transgenic plants were confirmed to be homoplastomic by PCR and Southern hybridization. ELISA assay indicated that bFGF represented approximately 0.1% of total soluble protein in the leaves of transplastomic tobacco plants. This is the first report of bFGF expression via chloroplast transformation in model plant, providing an additional option for the production of chloroplast-produced therapeutic proteins. View Full-Text
Share & Cite This Article
Wang, Y.-P.; Wei, Z.-Y.; Zhong, X.-F.; Lin, C.-J.; Cai, Y.-H.; Ma, J.; Zhang, Y.-Y.; Liu, Y.-Z.; Xing, S.-C. Stable Expression of Basic Fibroblast Growth Factor in Chloroplasts of Tobacco. Int. J. Mol. Sci. 2016, 17, 19.
Wang Y-P, Wei Z-Y, Zhong X-F, Lin C-J, Cai Y-H, Ma J, Zhang Y-Y, Liu Y-Z, Xing S-C. Stable Expression of Basic Fibroblast Growth Factor in Chloroplasts of Tobacco. International Journal of Molecular Sciences. 2016; 17(1):19.Chicago/Turabian Style
Wang, Yun-Peng; Wei, Zheng-Yi; Zhong, Xiao-Fang; Lin, Chun-Jing; Cai, Yu-Hong; Ma, Jian; Zhang, Yu-Ying; Liu, Yan-Zhi; Xing, Shao-Chen. 2016. "Stable Expression of Basic Fibroblast Growth Factor in Chloroplasts of Tobacco." Int. J. Mol. Sci. 17, no. 1: 19.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.