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Int. J. Mol. Sci. 2016, 17(1), 19; doi:10.3390/ijms17010019

Stable Expression of Basic Fibroblast Growth Factor in Chloroplasts of Tobacco

1
Jilin Provincial Key Laboratory of Agricultural Biotechnology, Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, No. 1363, Shengtai st., Changchun 130033, China
2
Institute of Agricultural Quality Standard and Testing Technology, Jilin Academy of Agricultural Sciences, No. 1363, Shengtai st., Changchun 130033, China
3
Faculty of Agronomy, Jilin Agricultural University, No. 2888, Xincheng st., Changchun 130118, China
4
College of Biological Sciences, China Agricultural University, No. 2 West Yuanmingyuan Road, Beijing 100094, China
*
Author to whom correspondence should be addressed.
Academic Editors: Setsuko Komatsu and Marcello Iriti
Received: 4 November 2015 / Revised: 30 November 2015 / Accepted: 15 December 2015 / Published: 23 December 2015
(This article belongs to the Special Issue Plant Proteomic Research)
View Full-Text   |   Download PDF [2198 KB, uploaded 23 December 2015]   |  

Abstract

Basic fibroblast growth factor (bFGF) is a multifunctional factor in acceleration of cell proliferation, differentiation and transference, and therefore widely used in clinical applications. In this study, expression vector pWX-Nt03 harboring a codon-optimized bFGF gene was constructed and introduced into the tobacco chloroplasts by particle bombardment. After four rounds of selection, bFGF was proved to integrate into the chloroplast genome of regenerated plants and two of four transgenic plants were confirmed to be homoplastomic by PCR and Southern hybridization. ELISA assay indicated that bFGF represented approximately 0.1% of total soluble protein in the leaves of transplastomic tobacco plants. This is the first report of bFGF expression via chloroplast transformation in model plant, providing an additional option for the production of chloroplast-produced therapeutic proteins. View Full-Text
Keywords: tobacco; chloroplast; genetic transformation; basic fibroblast growth factor; green fluorescent protein tobacco; chloroplast; genetic transformation; basic fibroblast growth factor; green fluorescent protein
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Wang, Y.-P.; Wei, Z.-Y.; Zhong, X.-F.; Lin, C.-J.; Cai, Y.-H.; Ma, J.; Zhang, Y.-Y.; Liu, Y.-Z.; Xing, S.-C. Stable Expression of Basic Fibroblast Growth Factor in Chloroplasts of Tobacco. Int. J. Mol. Sci. 2016, 17, 19.

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