K88 Fimbrial Adhesin Targeting of Microspheres Containing Gentamicin Made with Albumin Glycated with Lactose†
AbstractThe formulation and characterization of gentamicin-loaded microspheres as a delivery system targeting enterotoxigenic Escherichia coli K88 (E. coli K88) was investigated. Glycated albumin with lactose (BSA-glucose-β (4-1) galactose) was used as the microsphere matrix (MS-Lac) and gentamicin included as the transported antibiotic. The proposed target strategy was that exposed galactoses of MS-Lac could be specifically recognized by E. coli K88 adhesins, and the delivery of gentamicin would inhibit bacterial growth. Lactosylated microspheres (MS-Lac1, MS-Lac2 and MS-Lac3) were obtained using a water-in-oil emulsion, containing gentamicin, followed by crosslinking with different concentrations of glutaraldehyde. Electron microscopy displayed spherical particles with a mean size of 10–17 µm. In vitro release of gentamicin from MS-Lac was best fitted to a first order model, and the antibacterial activity of encapsulated and free gentamicin was comparable. MS-Lac treatments were recognized by plant galactose-specific lectins from Ricinus communis and Sophora japonica and by E. coli K88 adhesins. Results indicate MS-Lac1, produced with 4.2 mg/mL of crosslinker, as the best treatment and that lactosylated microsphere are promising platforms to obtain an active, targeted system against E. coli K88 infections. View Full-Text
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Sarabia-Sainz, A.-I.; Sarabia-Sainz, H.M.; Montfort, G.R.-C.; Mata-Haro, V.; Guzman-Partida, A.M.; Guzman, R.; Garcia-Soto, M.; Vazquez-Moreno, L. K88 Fimbrial Adhesin Targeting of Microspheres Containing Gentamicin Made with Albumin Glycated with Lactose. Int. J. Mol. Sci. 2015, 16, 22425-22437.
Sarabia-Sainz A-I, Sarabia-Sainz HM, Montfort GR-C, Mata-Haro V, Guzman-Partida AM, Guzman R, Garcia-Soto M, Vazquez-Moreno L. K88 Fimbrial Adhesin Targeting of Microspheres Containing Gentamicin Made with Albumin Glycated with Lactose. International Journal of Molecular Sciences. 2015; 16(9):22425-22437.Chicago/Turabian Style
Sarabia-Sainz, Andre-i; Sarabia-Sainz, Hector M.; Montfort, Gabriela R.-C.; Mata-Haro, Veronica; Guzman-Partida, Ana M.; Guzman, Roberto; Garcia-Soto, Mariano; Vazquez-Moreno, Luz. 2015. "K88 Fimbrial Adhesin Targeting of Microspheres Containing Gentamicin Made with Albumin Glycated with Lactose." Int. J. Mol. Sci. 16, no. 9: 22425-22437.