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Int. J. Mol. Sci. 2015, 16(9), 22425-22437; doi:10.3390/ijms160922425

K88 Fimbrial Adhesin Targeting of Microspheres Containing Gentamicin Made with Albumin Glycated with Lactose

1
Departamento de Investigacion en Fisica, Universidad de Sonora, Hermosillo Sonora 83000, Mexico
2
Laboratorio de Bioquimica de Proteinas y Glicanos, Coordinacion de Ciencia de los Alimentos, Centro de Investigacion en Alimentacion y Desarrollo A.C., Hermosillo Sonora 83304, Mexico
3
Departamento de Investigacion y Posgrado en Alimentos, Universidad de Sonora, Hermosillo Sonora 83000, Mexico
4
Department of Chemical and Environmental Engineering, the University of Arizona, Tucson, AZ 85721, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Már Másson
Received: 11 June 2015 / Revised: 7 August 2015 / Accepted: 7 August 2015 / Published: 16 September 2015
(This article belongs to the Special Issue Drug Delivery and Antimicrobial Agents)
View Full-Text   |   Download PDF [2151 KB, uploaded 16 September 2015]   |  

Abstract

The formulation and characterization of gentamicin-loaded microspheres as a delivery system targeting enterotoxigenic Escherichia coli K88 (E. coli K88) was investigated. Glycated albumin with lactose (BSA-glucose-β (4-1) galactose) was used as the microsphere matrix (MS-Lac) and gentamicin included as the transported antibiotic. The proposed target strategy was that exposed galactoses of MS-Lac could be specifically recognized by E. coli K88 adhesins, and the delivery of gentamicin would inhibit bacterial growth. Lactosylated microspheres (MS-Lac1, MS-Lac2 and MS-Lac3) were obtained using a water-in-oil emulsion, containing gentamicin, followed by crosslinking with different concentrations of glutaraldehyde. Electron microscopy displayed spherical particles with a mean size of 10–17 µm. In vitro release of gentamicin from MS-Lac was best fitted to a first order model, and the antibacterial activity of encapsulated and free gentamicin was comparable. MS-Lac treatments were recognized by plant galactose-specific lectins from Ricinus communis and Sophora japonica and by E. coli K88 adhesins. Results indicate MS-Lac1, produced with 4.2 mg/mL of crosslinker, as the best treatment and that lactosylated microsphere are promising platforms to obtain an active, targeted system against E. coli K88 infections. View Full-Text
Keywords: lactosylated microspheres; gentamicin; targeted active drug lactosylated microspheres; gentamicin; targeted active drug
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Sarabia-Sainz, A.-I.; Sarabia-Sainz, H.M.; Montfort, G.R.-C.; Mata-Haro, V.; Guzman-Partida, A.M.; Guzman, R.; Garcia-Soto, M.; Vazquez-Moreno, L. K88 Fimbrial Adhesin Targeting of Microspheres Containing Gentamicin Made with Albumin Glycated with Lactose. Int. J. Mol. Sci. 2015, 16, 22425-22437.

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