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Int. J. Mol. Sci. 2015, 16(9), 22008-22026; doi:10.3390/ijms160922008

Gene Expression of Type VI Secretion System Associated with Environmental Survival in Acidovorax avenae subsp. avenae by Principle Component Analysis

1
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China
2
Yuhang Extension and Service Center of Agriculture Technical, Hangzhou 311100, China
3
State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest and Disease Control, Key Laboratory of Detection for Pesticide Residues, Ministry of Agriculture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Patrick C.Y. Woo
Received: 8 June 2015 / Revised: 17 August 2015 / Accepted: 18 August 2015 / Published: 11 September 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [1457 KB, uploaded 11 September 2015]   |  

Abstract

Valine glycine repeat G (VgrG) proteins are regarded as one of two effectors of Type VI secretion system (T6SS) which is a complex multi-component secretion system. In this study, potential biological roles of T6SS structural and VgrG genes in a rice bacterial pathogen, Acidovorax avenae subsp. avenae (Aaa) RS-1, were evaluated under seven stress conditions using principle component analysis of gene expression. The results showed that growth of the pathogen was reduced by H2O2 and paraquat-induced oxidative stress, high salt, low temperature, and vgrG mutation, compared to the control. However, pathogen growth was unaffected by co-culture with a rice rhizobacterium Burkholderia seminalis R456. In addition, expression of 14 T6SS structural and eight vgrG genes was significantly changed under seven conditions. Among different stress conditions, high salt, and low temperature showed a higher effect on the expression of T6SS gene compared with host infection and other environmental conditions. As a first report, this study revealed an association of T6SS gene expression of the pathogen with the host infection, gene mutation, and some common environmental stresses. The results of this research can increase understanding of the biological function of T6SS in this economically-important pathogen of rice. View Full-Text
Keywords: T6SS gene expression; gene knockout; co-culture; in vivo; stress; principle component analysis T6SS gene expression; gene knockout; co-culture; in vivo; stress; principle component analysis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Cui, Z.; Jin, G.; Li, B.; Kakar, K.U.; Ojaghian, M.R.; Wang, Y.; Xie, G.; Sun, G. Gene Expression of Type VI Secretion System Associated with Environmental Survival in Acidovorax avenae subsp. avenae by Principle Component Analysis. Int. J. Mol. Sci. 2015, 16, 22008-22026.

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