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Int. J. Mol. Sci. 2015, 16(9), 22027-22045; doi:10.3390/ijms160922027

Size Does Matter: Staging of Silene latifolia Floral Buds for Transcriptome Studies

Department of Biology, Program on Disease Resistance, University of Louisville, Louisville, KY 40292, USA
Authors to whom correspondence should be addressed.
Academic Editor: Jan Schirawski
Received: 21 July 2015 / Revised: 30 August 2015 / Accepted: 6 September 2015 / Published: 11 September 2015
(This article belongs to the Special Issue Plant Microbe Interaction)
View Full-Text   |   Download PDF [6002 KB, uploaded 11 September 2015]   |  


Dioecious plants in the Caryophyllaceae family are susceptible to infection by members of the anthericolous smut fungi. In our studies of the Silene latifolia/Microbotryum lychnidis-dioicae pathosystem, we were interested in characterizing the plant-pathogen interaction at the molecular level before and during teliosporogenesis. This takes place during floral bud development, and we hoped to capture the interaction by Illumina Next-Gen RNA-Sequencing. Using previous literature that documented the stages of the floral buds for S. latifolia, we examined the floral buds from plants grown and infected under growth chamber conditions, using the disserting microscope to determine the stage of floral buds based on the morphology. We compiled the information and determined the size of floral buds that correspond to the desired stages of development for tissue collection, for the purpose of RNA-sequencing. This offers a practical approach for researchers who require a large number of floral buds/tissue categorized by stages of development, ascertaining whether infected/uninfected buds are at comparable stages of development and whether this also holds true for male vs. female buds. We also document our experience in infecting the plants and some of the unusual morphologies we observed after infection. View Full-Text
Keywords: Silene latifolia; Microbotryum; anther smut; RNA-Seq; floral bud stage; host-pathogen interactions Silene latifolia; Microbotryum; anther smut; RNA-Seq; floral bud stage; host-pathogen interactions

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Toh, S.S.; Perlin, M.H. Size Does Matter: Staging of Silene latifolia Floral Buds for Transcriptome Studies. Int. J. Mol. Sci. 2015, 16, 22027-22045.

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