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Int. J. Mol. Sci. 2015, 16(9), 21442-21453; doi:10.3390/ijms160921442

MicroRNA, Pm-miR-2305, Participates in Nacre Formation by Targeting Pearlin in Pearl Oyster Pinctada martensii

1
Fishery College, Guangdong Ocean University, Zhanjiang 524025, Guangdong, China
2
Guangdong Technology Research Center for Pearl Aquaculutre and Process, Guangdong Ocean University, Zhanjiang 524025, China
*
Author to whom correspondence should be addressed.
Academic Editor: Michael R. Ladomery
Received: 5 June 2015 / Revised: 16 August 2015 / Accepted: 26 August 2015 / Published: 7 September 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

MicroRNAs (miRNAs) are noncoding RNA molecules that function as negative regulators of target genes. In our previous research, 258 pm-miRNAs were identified in Pinctada martensii by Solexa deep sequencing. Pm-miR-2305 was one of the identified pm-miRNAs with a potential function in biomineralization. In the present study, the precursor of pm-miR-2305 was predicted with 96 bp, containing a characteristic hairpin structure. Stem-loop qRT-PCR analysis indicated that pm-miR-2305 was constitutively expressed in all the tissues (adductor muscle, gill, mantle, hepatopancreas, foot, and gonad) of P. martensii and was highly expressed in the foot. After the over-expression of pm-miR-2305 in the mantle by mimics injection into the muscle of P. martensii, nacre demonstrated disorderly growth, as detected by scanning electron microscopy. Dual luciferase reporter gene assay indicated that pm-miR-2305 mimics could significantly inhibit the luciferase activity of the reporter containing the 3′UTR of the pearlin gene. Western blot analysis demonstrated that the protein expression of pearlin was down-regulated in the mantle tissue after the over-expression of pm-miR-2305. Therefore, our data showed that pm-miR-2305 participated in nacre formation by targeting pearlin in P. martensii. View Full-Text
Keywords: Pinctada martensii; biomineralization; nacre formation; miR-2305 Pinctada martensii; biomineralization; nacre formation; miR-2305
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Jiao, Y.; Zheng, Z.; Tian, R.; Du, X.; Wang, Q.; Huang, R. MicroRNA, Pm-miR-2305, Participates in Nacre Formation by Targeting Pearlin in Pearl Oyster Pinctada martensii. Int. J. Mol. Sci. 2015, 16, 21442-21453.

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