The Essential Role of Vitellogenin Receptor in Ovary Development and Vitellogenin Uptake in Bactrocera dorsalis (Hendel)
Abstract
:1. Introduction
2. Results
2.1. Sequence and Structural Characteristics of VgR in B. dorsalis
2.2. Sequences Comparison and Phylogenetic Analysis
2.3. Tissue-Specific Expression Pattern of BdVgR
2.4. Ovary Growth, Developmental-Specific Expression Patterns of BdVgR, Bdyp1 and Bdyp2
2.5. Silencing BdVgR Expression by RNA Interference
3. Discussion
4. Experimental Section
4.1. Insects Rearing, Sample Preparation and Ovarian Diameter Measurement
4.2. RNA Extraction and Cloning of BdVgR
4.3. Bioinformatics Analysis of BdVgR
4.4. Semi-Quantitative PCR and Quantitative PCR (qPCR)
4.5. RNA Interference
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Cong, L.; Yang, W.-J.; Jiang, X.-Z.; Niu, J.-Z.; Shen, G.-M.; Ran, C.; Wang, J.-J. The Essential Role of Vitellogenin Receptor in Ovary Development and Vitellogenin Uptake in Bactrocera dorsalis (Hendel). Int. J. Mol. Sci. 2015, 16, 18368-18383. https://doi.org/10.3390/ijms160818368
Cong L, Yang W-J, Jiang X-Z, Niu J-Z, Shen G-M, Ran C, Wang J-J. The Essential Role of Vitellogenin Receptor in Ovary Development and Vitellogenin Uptake in Bactrocera dorsalis (Hendel). International Journal of Molecular Sciences. 2015; 16(8):18368-18383. https://doi.org/10.3390/ijms160818368
Chicago/Turabian StyleCong, Lin, Wen-Jia Yang, Xuan-Zhao Jiang, Jin-Zhi Niu, Guang-Mao Shen, Chun Ran, and Jin-Jun Wang. 2015. "The Essential Role of Vitellogenin Receptor in Ovary Development and Vitellogenin Uptake in Bactrocera dorsalis (Hendel)" International Journal of Molecular Sciences 16, no. 8: 18368-18383. https://doi.org/10.3390/ijms160818368