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Int. J. Mol. Sci. 2015, 16(8), 17231-17244; doi:10.3390/ijms160817231

Mapping of Powdery Mildew Resistance Gene pmCH89 in a Putative Wheat-Thinopyrum intermedium Introgression Line

1
College of Life Science, Shanxi University, Taiyuan 030006, Shanxi, China
2
Institute of Crop Science, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, Shanxi, China
3
Key Laboratory for Crop Gene Resources and Germplasm Enhancement on the Loess Plateau, Ministry of Agriculture, Taiyuan 030031, Shanxi, China
4
College of Agronomy, Shanxi Agricultural University, Taigu 030801, Shanxi, China
*
Author to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Received: 1 April 2015 / Revised: 22 July 2015 / Accepted: 22 July 2015 / Published: 28 July 2015
(This article belongs to the Special Issue Plant Molecular Biology)
View Full-Text   |   Download PDF [2586 KB, uploaded 28 July 2015]   |  

Abstract

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a globally serious disease adversely affecting wheat production. The Bgt-resistant wheat breeding line CH09W89 was derived after backcrossing a Bgt resistant wheat-Thinopyrum intermedium partial amphiploid TAI7045 with susceptible wheat cultivars. At the seedling stage, CH09W89 exhibited immunity or high resistance to Bgt pathotypes E09, E20, E21, E23, E26, Bg1, and Bg2, similar to its donor line TAI7045 and Th. intermedium. No Th. intermedium chromatin was detected based on genomic in situ hybridization of mitotic chromosomes. To determine the mode of inheritance of the Bgt resistance and the chromosomal location of the resistance gene, CH09W89 was crossed with two susceptible wheat cultivars. The results of the genetic analysis showed that the adult resistance to Bgt E09 in CH09W89 was controlled by a single recessive gene, which was tentatively designated as pmCH89. Two polymorphic SSR markers, Xwmc310 and Xwmc125, were linked to the resistance gene with genetic distances 3.1 and 2.7 cM, respectively. Using the Chinese Spring aneuploid and deletion lines, the resistance gene and its linked markers were assigned to chromosome arm 4BL in the bin 0.68–0.78. Due to its unique position on chromosome 4BL, pmCH89 appears to be a new locus for resistance to powdery mildew. These results will be of benefit for improving powdery mildew resistance in wheat breeding programs. View Full-Text
Keywords: wheat-Thinopyrum intermedium introgression line; Th. intermedium; powdery mildew; genetic analysis; molecular mapping wheat-Thinopyrum intermedium introgression line; Th. intermedium; powdery mildew; genetic analysis; molecular mapping
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MDPI and ACS Style

Hou, L.; Zhang, X.; Li, X.; Jia, J.; Yang, H.; Zhan, H.; Qiao, L.; Guo, H.; Chang, Z. Mapping of Powdery Mildew Resistance Gene pmCH89 in a Putative Wheat-Thinopyrum intermedium Introgression Line. Int. J. Mol. Sci. 2015, 16, 17231-17244.

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