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Int. J. Mol. Sci. 2015, 16(7), 16655-16668; doi:10.3390/ijms160716655

Fluoxetine Decreases the Proliferation and Adipogenic Differentiation of Human Adipose-Derived Stem Cells

1
College of Pharmacy, Yonsei University, Incheon 406-840, Korea
2
STEMORE Co., Ltd., Incheon 406-840, Korea
3
College of Pharmacy, Catholic University of Daegu, Daegu 712-702, Korea
*
Authors to whom correspondence should be addressed.
Academic Editor: Toshiro Arai
Received: 16 June 2015 / Accepted: 14 July 2015 / Published: 22 July 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [1914 KB, uploaded 22 July 2015]   |  

Abstract

Fluoxetine was originally developed as an antidepressant, but it has also been used to treat obesity. Although the anti-appetite effect of fluoxetine is well-documented, its potential effects on human adipose-derived stem cells (ASCs) or mature adipocytes have not been investigated. Therefore, we investigated the mechanisms underlying the inhibitory effects of fluoxetine on the proliferation of ASCs. We also investigated its inhibitory effect on adipogenic differentiation. Fluoxetine significantly decreased ASC proliferation, and signal transduction PCR array analysis showed that it increased expression of autophagy-related genes. In addition, fluoxetine up-regulated SQSTM1 and LC3B protein expression as detected by western blotting and immunofluorescence. The autophagy inhibitor, 3-methyladenine (3-MA), significantly attenuated fluoxetine-mediated effects on ASC proliferation and SQSTM1/LC3B expression. In addition, 3-MA decreased the mRNA expression of two autophagy-related genes, beclin-1 and Atg7, in ASCs. Fluoxetine also significantly inhibited lipid accumulation and down-regulated the levels of PPAR-γ and C/EBP-α in ASCs. Collectively, these results indicate that fluoxetine decreases ASC proliferation and adipogenic differentiation. This is the first in vitro evidence that fluoxetine can reduce fat accumulation by inhibiting ASC proliferation and differentiation. View Full-Text
Keywords: adipose-derived stem cells; fluoxetine; autophagy; proliferation; adipogenic differentiation adipose-derived stem cells; fluoxetine; autophagy; proliferation; adipogenic differentiation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Sun, B.K.; Kim, J.H.; Choi, J.-S.; Hwang, S.-J.; Sung, J.-H. Fluoxetine Decreases the Proliferation and Adipogenic Differentiation of Human Adipose-Derived Stem Cells. Int. J. Mol. Sci. 2015, 16, 16655-16668.

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