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Int. J. Mol. Sci. 2015, 16(7), 15560-15577; doi:10.3390/ijms160715560

Purification and Structural Identification of Polysaccharides from Bamboo Shoots (Dendrocalamus latiflorus)

1
College of Food Science, Southwest University, Chongqing 400715, China
2
Chongqing Key Laboratory of Agricultural Product Processing, Southwest University, Chongqing 400715, China
*
Author to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Received: 20 April 2015 / Revised: 24 June 2015 / Accepted: 25 June 2015 / Published: 9 July 2015
(This article belongs to the Special Issue Plant Molecular Biology)
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Abstract

Three kinds of polysaccharides, namely, BSP1A, BSP2A, and BSP3B, were isolated from raw bamboo shoot (Dendrocalamus latiflorus) after purification and classification by DEAE cellulose-52 (ion-exchange chromatography) and Sephadex G-50. The molecular weights of BSP1A, BSP2A, and BSP3B were 10.2, 17.0 and 20.0 kDa, respectively, which were measured through GPC (gel performance chromtatography) methods. BSP1A contained arabinose, glucose, and galactose in a molar ratio of 1.0:40.6:8.7. BSP2A and BSP3B contained arabinose, xylose, glucose, and galactose in molar ratios of 6.6:1.0:5.2:10.4 and 8.5:1.0:5.1:11.1, respectively. The existence of the O-glycopeptide bond in BSP1A, BSP2A, and BSP3B was demonstrated by β-elimination reaction. FTIR spectra of the three polysaccharides showed that both BSP2A and BSP3B contained β-d-pyranose sugar rings. However, BSP1A exhibited both β-d-pyranose and α-d-pyranose sugar rings. Congo red test indicated that BSP1A and BSP2A displayed triple helix structures, but BSP3B did not. NMR spectroscopy revealed that BSP1A may exhibit a β-1,6-Glucan pyran type as the main link, and few 1,6-glycosidic galactose pyranose and arabinose bonds were connected; BSP2A mainly demonstrated →5)β-Ara(1→and→3)β-Gal(1→connection. Furthermore, BSP3B mainly presented →3)β-Glu(1→and→3)β-Gal(1→connection and may also contain few other glycosidic bonds. View Full-Text
Keywords: Dendrocalamus latiflorus; purification; structural identification Dendrocalamus latiflorus; purification; structural identification
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MDPI and ACS Style

Wu, J.; Zheng, J.; Xia, X.; Kan, J. Purification and Structural Identification of Polysaccharides from Bamboo Shoots (Dendrocalamus latiflorus). Int. J. Mol. Sci. 2015, 16, 15560-15577.

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