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Int. J. Mol. Sci. 2015, 16(6), 12076-12091; doi:10.3390/ijms160612076

Human Adipose-Derived Mesenchymal Progenitor Cells Engraft into Rabbit Articular Cartilage

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1
Cellular Biomedicine Group, Palo Alto, CA 94301, USA
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Department of Pathology, Shanghai Medical College, Fudan University, Shanghai 200433, China
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Animal facility, Shanghai Medical College, Fudan University, Shanghai 200433, China
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Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
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Department of Histology and Embryology, Shanghai Medical College, Fudan University, Shanghai 200433, China
Cellular Biomedicine Group, Shanghai 200233, China
*
Author to whom correspondence should be addressed.
Academic Editor: Wilhelm Bloch
Received: 26 March 2015 / Revised: 27 April 2015 / Accepted: 7 May 2015 / Published: 27 May 2015
(This article belongs to the Special Issue Stem Cell Activation in Adult Organism)
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Abstract

Mesenchymal stem cells (MSCs) are known to have the potential for articular cartilage regeneration, and are suggested for the treatment of osteoarthritis (OA). Here, we investigated whether intra-articular injection of xenogeneic human adipose-derived mesenchymal progenitor cells (haMPCs) promoted articular cartilage repair in rabbit OA model and engrafted into rabbit articular cartilage. The haMPCs were cultured in vitro, and phenotypes and differentiation characteristics of cells were evaluated. OA was induced surgically by anterior cruciate ligament transection (ACLT) and medical meniscectomy of knee joints. At six weeks following surgery, hyaluronic acid (HA) or haMPCs was injected into the knee joints, the contralateral knee served as normal control. All animals were sacrificed at the 16th week post-surgery. Assessments were carried out by macroscopic examination, hematoxylin/eosin (HE) and Safranin-O/Fast green stainings and immunohistochemistry. The data showed that haMPC treatment promoted cartilage repair. Signals of human mitochondrial can be directly detected in haMPC treated cartilage. The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo. These results suggest that intra-articular injection of haMPCs promotes regeneration of articular cartilage in rabbit OA model, and support the notion that MPCs are transplantable between HLA-incompatible individuals. View Full-Text
Keywords: mesenchymal stem cells; human adipose-derived mesenchymal progenitor cells; osteoarthritis; human leukocyte antigen mesenchymal stem cells; human adipose-derived mesenchymal progenitor cells; osteoarthritis; human leukocyte antigen
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Wang, W.; He, N.; Feng, C.; Liu, V.; Zhang, L.; Wang, F.; He, J.; Zhu, T.; Wang, S.; Qiao, W.; Li, S.; Zhou, G.; Zhang, L.; Dai, C.; Cao, W. Human Adipose-Derived Mesenchymal Progenitor Cells Engraft into Rabbit Articular Cartilage. Int. J. Mol. Sci. 2015, 16, 12076-12091.

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