Next Article in Journal
Soy and Breast Cancer: Focus on Angiogenesis
Next Article in Special Issue
Homo-FRET Based Biosensors and Their Application to Multiplexed Imaging of Signalling Events in Live Cells
Previous Article in Journal
MicroRNA-24 Regulates Osteogenic Differentiation via Targeting T-Cell Factor-1
Previous Article in Special Issue
Investigation of Förster Resonance Energy Transfer (FRET) and Competition of Fluorescent Dyes on DNA Microparticles
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2015, 16(5), 11713-11727; doi:10.3390/ijms160511713

Intravital FRET: Probing Cellular and Tissue Function in Vivo

1
Neuropathology, Charité–University of Medicine, Berlin 10117, Germany
2
Germany German Rheumatism Research Center, Berlin 10117, Germany
3
Immundynamics and Intravital Microscopy, Charité–University of Medicine, Berlin 10117, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Herbert Schneckenburger
Received: 20 February 2015 / Accepted: 13 May 2015 / Published: 21 May 2015
(This article belongs to the Special Issue Förster Resonance Energy Transfer (FRET) 2015)
View Full-Text   |   Download PDF [3618 KB, uploaded 21 May 2015]   |  

Abstract

The development of intravital Förster Resonance Energy Transfer (FRET) is required to probe cellular and tissue function in the natural context: the living organism. Only in this way can biomedicine truly comprehend pathogenesis and develop effective therapeutic strategies. Here we demonstrate and discuss the advantages and pitfalls of two strategies to quantify FRET in vivo—ratiometrically and time-resolved by fluorescence lifetime imaging—and show their concrete application in the context of neuroinflammation in adult mice. View Full-Text
Keywords: intravital FRET; multi-photon microscopy; fluorescence lifetime imaging; genetically encoded calcium indicators intravital FRET; multi-photon microscopy; fluorescence lifetime imaging; genetically encoded calcium indicators
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Radbruch, H.; Bremer, D.; Mothes, R.; Günther, R.; Rinnenthal, J.L.; Pohlan, J.; Ulbricht, C.; Hauser, A.E.; Niesner, R. Intravital FRET: Probing Cellular and Tissue Function in Vivo. Int. J. Mol. Sci. 2015, 16, 11713-11727.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top