MicroRNA-24 Regulates Osteogenic Differentiation via Targeting T-Cell Factor-1
AbstractMicroRNAs (miRNAs) have been reported to have diverse biological roles in regulating many biological processes, including osteogenic differentiation. In the present study, we identified that miR-24 was a critical regulator during osteogenic differentiation. We found that overexpression of miR-24 significantly inhibited osteogenic differentiation, which decreased alkaline phosphatase activity, matrix mineralization and the expression of osteogenic differentiation markers. In contrast, inhibition of miR-24 exhibited an opposite effect. Furthermore, we delineated that miR-24 regulates post-transcriptionals of T-cell factor-1 (Tcf-1) via targeting the 3'-untranslated region (UTR) of Tcf-1 mRNA. MiR-24 was further found to regulate the protein expression of Tcf-1 in the murine osteoprogenitors cells and bone mesenchymal stem cells. Additionally, the positive effect of miR-24 suppression on osteoblast differentiation was apparently abrogated by Tcf-1 silencing. Taken together, our data suggest that miR-24 participates in osteogenic differentiation by targeting and regulating Tcf-1 expression in osteoblastic cells. View Full-Text
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Zhao, W.; Wu, C.; Dong, Y.; Ma, Y.; Jin, Y.; Ji, Y. MicroRNA-24 Regulates Osteogenic Differentiation via Targeting T-Cell Factor-1. Int. J. Mol. Sci. 2015, 16, 11699-11712.
Zhao W, Wu C, Dong Y, Ma Y, Jin Y, Ji Y. MicroRNA-24 Regulates Osteogenic Differentiation via Targeting T-Cell Factor-1. International Journal of Molecular Sciences. 2015; 16(5):11699-11712.Chicago/Turabian Style
Zhao, Weigong; Wu, Caijun; Dong, Yanying; Ma, Yunfeng; Jin, Yaofeng; Ji, Yanhong. 2015. "MicroRNA-24 Regulates Osteogenic Differentiation via Targeting T-Cell Factor-1." Int. J. Mol. Sci. 16, no. 5: 11699-11712.