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Int. J. Mol. Sci. 2015, 16(4), 7738-7747; doi:10.3390/ijms16047738

Investigation of Förster Resonance Energy Transfer (FRET) and Competition of Fluorescent Dyes on DNA Microparticles

Department of Chemical Engineering, University of Seoul, Seoul 130-743, Korea
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Author to whom correspondence should be addressed.
Academic Editor: Herbert Schneckenburger
Received: 13 February 2015 / Revised: 27 March 2015 / Accepted: 27 March 2015 / Published: 8 April 2015
(This article belongs to the Special Issue Förster Resonance Energy Transfer (FRET) 2015)
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Abstract

Fluorescent labeling is widely used to investigate the structural stability and changes to DNA nano- and microstructures. Despite this, the conventional method for observing DNA structures has several limitations in terms of cost-efficiency. This paper introduces a DNA spherical particle stained with DNA intercalating dyes (SYBR Green and SYTOX Orange) as tracers and reports the interaction between multiple dyes. The interference between the dyes was analyzed in terms of Förster resonance energy transfer (FRET) and competition. The changes in the fluorescence intensity by FRET were uniform, regardless of the sequence. The competition effect could occur when several dyes were added simultaneously. These properties are expected to help in the design of multicolor tracers in bioimaging and environmental applications. View Full-Text
Keywords: DNA technology; DNA microparticles; bioimaging; Förster resonance energy transfer (FRET) DNA technology; DNA microparticles; bioimaging; Förster resonance energy transfer (FRET)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kim, J.; Lee, J.S.; Lee, J.B. Investigation of Förster Resonance Energy Transfer (FRET) and Competition of Fluorescent Dyes on DNA Microparticles. Int. J. Mol. Sci. 2015, 16, 7738-7747.

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