Effects of Paeonol on Anti-Neuroinflammatory Responses in Microglial Cells
Abstract
:1. Introduction
2. Results
2.1. Paeonol Suppresses LPS/IFN-γ-Induced Inflammatory Responses in Microglia
2.2. Paeonol Inhibits Migratory Activity and ROS Production in Microglial Cells
2.3. Anti-Neuroinflammatory Effects of Paeonol through AMPK Signaling Pathway
2.4. Paeonol-Induced Anti-Neuroinflammation is Mediated by GSK3α/β Activation
2.5. Effects of Paeonol on LPS-Induced Impairment of Motor Coordination and Microglial Activation
3. Discussion
4. Experimental Section
4.1. Reagents and Antibodies
4.2. Cell Culture
4.3. Animals
4.4. Western Blot Analysis
4.5. Migration Assay
4.6. Reactive Oxygen Species (ROS) Assay
4.7. Sickness-Like Behaviors
4.8. Immunohistochemical Analysis
4.9. Measurement of Cell Viability
4.10. Statistical Analyses
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Lin, C.; Lin, H.-Y.; Chen, J.-H.; Tseng, W.-P.; Ko, P.-Y.; Liu, Y.-S.; Yeh, W.-L.; Lu, D.-Y. Effects of Paeonol on Anti-Neuroinflammatory Responses in Microglial Cells. Int. J. Mol. Sci. 2015, 16, 8844-8860. https://doi.org/10.3390/ijms16048844
Lin C, Lin H-Y, Chen J-H, Tseng W-P, Ko P-Y, Liu Y-S, Yeh W-L, Lu D-Y. Effects of Paeonol on Anti-Neuroinflammatory Responses in Microglial Cells. International Journal of Molecular Sciences. 2015; 16(4):8844-8860. https://doi.org/10.3390/ijms16048844
Chicago/Turabian StyleLin, Chingju, Hsiao-Yun Lin, Jia-Hong Chen, Wen-Pei Tseng, Pei-Ying Ko, Yu-Shu Liu, Wei-Lan Yeh, and Dah-Yuu Lu. 2015. "Effects of Paeonol on Anti-Neuroinflammatory Responses in Microglial Cells" International Journal of Molecular Sciences 16, no. 4: 8844-8860. https://doi.org/10.3390/ijms16048844