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Int. J. Mol. Sci. 2015, 16(4), 8490-8504; doi:10.3390/ijms16048490

1H NMR-Based Metabolic Profiling Reveals the Effects of Fluoxetine on Lipid and Amino Acid Metabolism in Astrocytes

1,2,3,4,†
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2,3,†
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2,3,†
,
2,3,†
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2,3
,
5
,
1,2,3,4
,
2,3
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2,3
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and
1,2,3,6,*
1
Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, China
2
Chongqing Key Laboratory of Neurobiology, Chongqing 400016, China
3
Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China
4
Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China
5
Department of Neurology, Xinqiao Hospital, Third Military Medical University, Chongqing 400016, China
6
Department of Neurology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Bing Yan
Received: 3 February 2015 / Revised: 2 April 2015 / Accepted: 8 April 2015 / Published: 15 April 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [2348 KB, uploaded 15 April 2015]   |  

Abstract

Fluoxetine, a selective serotonin reuptake inhibitor (SSRI), is a prescribed and effective antidepressant and generally used for the treatment of depression. Previous studies have revealed that the antidepressant mechanism of fluoxetine was related to astrocytes. However, the therapeutic mechanism underlying its mode of action in astrocytes remains largely unclear. In this study, primary astrocytes were exposed to 10 µM fluoxetine; 24 h post-treatment, a high-resolution proton nuclear magnetic resonance (1H NMR)-based metabolomic approach coupled with multivariate statistical analysis was used to characterize the metabolic variations of intracellular metabolites. The orthogonal partial least-squares discriminant analysis (OPLS-DA) score plots of the spectra demonstrated that the fluoxetine-treated astrocytes were significantly distinguished from the untreated controls. In total, 17 differential metabolites were identified to discriminate the two groups. These key metabolites were mainly involved in lipids, lipid metabolism-related molecules and amino acids. This is the first study to indicate that fluoxetine may exert antidepressant action by regulating the astrocyte’s lipid and amino acid metabolism. These findings should aid our understanding of the biological mechanisms underlying fluoxetine therapy. View Full-Text
Keywords: fluoxetine; SSRI; astrocytes; depression; metabolomics; 1H NMR fluoxetine; SSRI; astrocytes; depression; metabolomics; 1H NMR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Bai, S.; Zhou, C.; Cheng, P.; Fu, Y.; Fang, L.; Huang, W.; Yu, J.; Shao, W.; Wang, X.; Liu, M.; Zhou, J.; Xie, P. 1H NMR-Based Metabolic Profiling Reveals the Effects of Fluoxetine on Lipid and Amino Acid Metabolism in Astrocytes. Int. J. Mol. Sci. 2015, 16, 8490-8504.

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