Molecular Characterization of Sec2 Loci in Wheat—Secale africanum Derivatives Demonstrates Genomic Divergence of Secale Species
Abstract
:1. Introduction
2. Results
2.1. Identification of Wheat—S. africanum Amphiploid and Substitution
2.2. Localization of Sec2 in the S. africanum Chromosome
2.3. The Sequence Homology of Sec2 Genes
Materials | Full-ORF | Pseudogenes |
---|---|---|
CSDA2R | JX877767 (1434 bp, 54.19 kD), JX877768 (1416 bp, 53.58 kD), JX877769 (1416 bp, 53.42 kD), JX877770 (1416 bp, 53.58 kD), JX877771 (1395 bp, 52.64 kD), JX877772 (1215 bp, 45.73 kD), JX877773 (1416 bp, 53.58 kD), JX877774 (1434 bp, 54.22 kD), JX877775 (1395 bp, 52.64 kD), JX877776 (1395 bp, 52.64 kD) | |
S. sylvestre | JX877738 (1503 bp, 56.73 kD), JX877739 (1503 bp, 56.71 kD), JX877740 (1503 bp, 56.71 kD), JX877741 (1503 bp, 56.71 kD), JX877742 (1203 bp, 45.03 kD), JX877743 (1503 bp, 56.70 kD), JX877744 (1503 bp, 56.58 kD), JX877745 (1503 bp, 56.71 kD), JX877746 (1503 bp, 56.73 kD) | |
YF-1 | JX877747 (1428 bp, 53.98 kD), JX877748 (1428 bp, 54.03 kD), JX877749 (1260 bp, 47.38 kD), JX877750 (1260 bp, 47.38 kD) | JX877751(1371 bp), JX877752(1356 bp), JX877753 (1356 bp), JX877754 (1356 bp), JX877755 (1356 bp) |
HH41 | JX877756 (1428 bp, 54.39 kD), JX877757 (1464 bp, 55.23 kD), JX877758 (1428 bp, 53.90 kD), JX877759 (1452 bp, 54.64 kD), JX877760 (1311 bp, 49.04 kD) | JX877761 (1320 bp), JX877762( 1332 bp), JX877763 (1332 bp), JX877764 (1332 bp), JX877765 (1320 bp), JX877766 (1332 bp) |
2.4. The Amino Acid Sequences of Sec2 Genes
2.5. Phylogenetic Tree and the Evolutionary Analyses
3. Discussion
4. Experimental Section
4.1. Plant Materials
4.2. Genomic in Situ Hybridization and Fluorescence in Situ Hybridization
4.3. Primer Design, PCR Cloning and Sequencing
4.4. Phylogenetic Analyses
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Li, G.; Zhang, H.; Zhou, L.; Gao, D.; Lei, M.; Zhang, J.; Yang, Z. Molecular Characterization of Sec2 Loci in Wheat—Secale africanum Derivatives Demonstrates Genomic Divergence of Secale Species. Int. J. Mol. Sci. 2015, 16, 8324-8336. https://doi.org/10.3390/ijms16048324
Li G, Zhang H, Zhou L, Gao D, Lei M, Zhang J, Yang Z. Molecular Characterization of Sec2 Loci in Wheat—Secale africanum Derivatives Demonstrates Genomic Divergence of Secale Species. International Journal of Molecular Sciences. 2015; 16(4):8324-8336. https://doi.org/10.3390/ijms16048324
Chicago/Turabian StyleLi, Guangrong, Hongjun Zhang, Li Zhou, Dan Gao, Mengping Lei, Jie Zhang, and Zujun Yang. 2015. "Molecular Characterization of Sec2 Loci in Wheat—Secale africanum Derivatives Demonstrates Genomic Divergence of Secale Species" International Journal of Molecular Sciences 16, no. 4: 8324-8336. https://doi.org/10.3390/ijms16048324