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Int. J. Mol. Sci. 2015, 16(4), 8310-8323; doi:10.3390/ijms16048310

Selection of Reference Genes for MicroRNA Quantitative Expression Analysis in Chinese Perch, Siniperca chuatsi

1
Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China
2
College of Veterinary Medicine, Hunan Agriculture University, Changsha 410128, China
3
Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde 415000, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Li Lin
Received: 6 February 2015 / Revised: 30 March 2015 / Accepted: 31 March 2015 / Published: 14 April 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [1410 KB, uploaded 14 April 2015]   |  

Abstract

Real-time quantitative reverse transcription PCR (RT-qPCR) is one of the most effective and sensitive techniques in gene expression assay, for which selection of reference genes is a prerequisite. In teleost species, such as Chinese perch, the expression profiling of miRNAs as reference genes for RT-qPCR has not been intensively studied. In the present study, the expression profiles of six miRNAs (miR-101a, miR-146a, miR-22a, miR-23a, miR-26a and let-7a) and one small nuclear RNA (U6) were assayed with RT-qPCR in different adult tissues, developmental stages and growth conditions of Chinese perch, Siniperca chuatsi. The analyses revealed that embryonic developmental stage is an important variability factor in the expression stability of miRNAs. All six miRNAs exhibited better expression consistency than U6 in most of the conditions examined, and therefore, they may be more suitable as a reference gene for miRNA quantification. When different tissues and developmental stages were considered, miR-22a demonstrated the most consistent expression pattern, and the best combination of reference genes was miR-22a and miR-23a. Our study offers useful data for selecting miRNAs as reference genes for RT-qPCR analysis of miRNAs in teleost fishes under different conditions. View Full-Text
Keywords: miRNA; real-time PCR; reference genes; relative quantification; Siniperca chuatsi miRNA; real-time PCR; reference genes; relative quantification; Siniperca chuatsi
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zhu, X.; Li, Y.-L.; Chen, D.-X.; Wu, P.; Yi, T.; Chen, T.; Zhang, J.-S.; Chu, W.-Y. Selection of Reference Genes for MicroRNA Quantitative Expression Analysis in Chinese Perch, Siniperca chuatsi. Int. J. Mol. Sci. 2015, 16, 8310-8323.

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