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Int. J. Mol. Sci. 2015, 16(4), 7320-7333; doi:10.3390/ijms16047320

Enhancement of Phenol Biodegradation by Pseudochrobactrum sp. through Ultraviolet-Induced Mutation

School of Environment Science and Spatial Informatics, China University of Mining and Technology, Xuzhou 221008, China
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Author to whom correspondence should be addressed.
Academic Editor: Daniel Rittschof
Received: 8 October 2014 / Revised: 16 March 2015 / Accepted: 20 March 2015 / Published: 1 April 2015
(This article belongs to the Section Green Chemistry)
View Full-Text   |   Download PDF [2543 KB, uploaded 1 April 2015]   |  

Abstract

The phenol-degrading efficiency of Pseudochrobactrum sp. was enhanced by ultraviolet (UV) irradiation. First, a bacterial strain, Pseudochrobactrum sp. XF1, was isolated from the activated sludge in a coking plant. It was subjected to mutation by UV radiation for 120 s and a mutant strain with higher phenol-degrading efficiency, Pseudochrobactrum sp. XF1-UV, was selected. The mutant strain XF1-UV was capable of degrading 1800 mg/L phenol completely within 48 h and had higher tolerance to hydrogen ion concentration and temperature variation than the wild type. Haldane’s kinetic model was used to fit the exponential growth data and the following kinetic parameters were obtained: μmax = 0.092 h−1, Ks = 22.517 mg/L, and Ki = 1126.725 mg/L for XF1, whereas μmax = 0.110 h−1, Ks = 23.934 mg/L, and Ki = 1579.134 mg/L for XF1-UV. Both XF1 and XF1-UV degraded phenol through the ortho-pathway; but the phenol hydroxylase activity of XF1-UV1 was higher than that of XF1, therefore, the mutant strain biodegraded phenol faster. Taken together, our results suggest that Pseudochrobactrum sp. XF1-UV could be a promising candidate for bioremediation of phenol-containing wastewaters. View Full-Text
Keywords: biodegradation; degradation kinetics; mutant; phenol; Pseudochrobactrum sp. biodegradation; degradation kinetics; mutant; phenol; Pseudochrobactrum sp.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Mao, Z.; Yu, C.; Xin, L. Enhancement of Phenol Biodegradation by Pseudochrobactrum sp. through Ultraviolet-Induced Mutation. Int. J. Mol. Sci. 2015, 16, 7320-7333.

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