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Int. J. Mol. Sci. 2015, 16(4), 6631-6644; doi:10.3390/ijms16046631

A Soluble Pyrophosphatase Is Essential to Oogenesis and Is Required for Polyphosphate Metabolism in the Red Flour Beetle (Tribolium castaneum)

1
Laboratory of Integrated Biochemistry—Hatisaburo Masuda, Universidade Federal do Rio de Janeiro, Núcleo em Ecologia e Desenvolvimento Sócio Ambiental de Macaé, Avenida São José do Barreto, 764, São José do Barreto, Macaé, RJ CEP 27965-045, Brazil
2
Nacional Institute of Science and Technology—Molecular Entomology, Rio de Janeiro, RJ CEP 21941-590, Brazil
3
Laboratory of Chemistry and Function of Proteins and Peptides and Unity of Animal Experimentation, Biotecnology and Bioscience Center, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego, 2000, Horto, Campos dos Goytacazes, RJ CEP 28015-620, Brazil
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Hefeng Huang
Received: 8 January 2015 / Revised: 13 February 2015 / Accepted: 9 March 2015 / Published: 24 March 2015
(This article belongs to the Special Issue Advances in Reproductive Biology)
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Abstract

Polyphosphates have been found in all cell types examined to date and play diverse roles depending on the cell type. In eukaryotic organisms, polyphosphates have been mainly investigated in mammalian cells with few studies on insects. Some studies have demonstrated that a pyrophosphatase regulates polyphosphate metabolism, and most of them were performed on trypanosomatids. Here, we investigated the effects of sPPase gene knocked down in oogenesis and polyphosphate metabolism in the red flour beetle (Tribolium castaneum). A single sPPase gene was identified in insect genome and is maternally provided at the mRNA level and not restricted to any embryonic or extraembryonic region during embryogenesis. After injection of Tc-sPPase dsRNA, female survival was reduced to 15% of the control (dsNeo RNA), and egg laying was completely impaired. The morphological analysis by nuclear DAPI staining of the ovarioles in Tc-sPPase dsRNA-injected females showed that the ovariole number is diminished, degenerated oocytes can be observed, and germarium is reduced. The polyphosphate level was increased in cytoplasmic and nuclear fractions in Tc-sPPase RNAi; Concomitantly, the exopolyphosphatase activity decreased in both fractions. Altogether, these data suggest a role for sPPase in the regulation on polyphosphate metabolism in insects and provide evidence that Tc-sPPase is essential to oogenesis. View Full-Text
Keywords: polyphosphate; pyrophosphatase; oogenesis; embryogenesis; insect polyphosphate; pyrophosphatase; oogenesis; embryogenesis; insect
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Carvalho, K.; Ribeiro, L.; Moraes, J.; da Silva, J.R.; Costa, E.P.; Souza-Menezes, J.; Logullo, C.; da Fonseca, R.N.; Campos, E. A Soluble Pyrophosphatase Is Essential to Oogenesis and Is Required for Polyphosphate Metabolism in the Red Flour Beetle (Tribolium castaneum). Int. J. Mol. Sci. 2015, 16, 6631-6644.

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