A Soluble Pyrophosphatase Is Essential to Oogenesis and Is Required for Polyphosphate Metabolism in the Red Flour Beetle (Tribolium castaneum)
Abstract
:1. Introduction
2. Results and Discussion
2.1. A Single Soluble Inorganic Pyrophosphatases (sPPases) Can Be Identified in Tribolium castaneum and Other Insect Genomes
2.2. pRNAi Analysis Shows that Tc-sPPase Is Essential to Oogenesis and Regulates Poly P Metabolism
3. Experimental Section
3.1. Tribolium castaneum Strains
3.2. Sequence Analysis
3.3. Primer Design, in Situ Hybridization and RNAi
3.4. Real-Time PCR
3.5. Cell Fractionation
3.6. Exopolyphosphatase Activity
3.7. Poly P Extraction and Quantification
3.8. Statistical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Carvalho, K.; Ribeiro, L.; Moraes, J.; Da Silva, J.R.; Costa, E.P.; Souza-Menezes, J.; Logullo, C.; Da Fonseca, R.N.; Campos, E. A Soluble Pyrophosphatase Is Essential to Oogenesis and Is Required for Polyphosphate Metabolism in the Red Flour Beetle (Tribolium castaneum). Int. J. Mol. Sci. 2015, 16, 6631-6644. https://doi.org/10.3390/ijms16046631
Carvalho K, Ribeiro L, Moraes J, Da Silva JR, Costa EP, Souza-Menezes J, Logullo C, Da Fonseca RN, Campos E. A Soluble Pyrophosphatase Is Essential to Oogenesis and Is Required for Polyphosphate Metabolism in the Red Flour Beetle (Tribolium castaneum). International Journal of Molecular Sciences. 2015; 16(4):6631-6644. https://doi.org/10.3390/ijms16046631
Chicago/Turabian StyleCarvalho, Klébea, Lupis Ribeiro, Jorge Moraes, José Roberto Da Silva, Evenilton P. Costa, Jackson Souza-Menezes, Carlos Logullo, Rodrigo Nunes Da Fonseca, and Eldo Campos. 2015. "A Soluble Pyrophosphatase Is Essential to Oogenesis and Is Required for Polyphosphate Metabolism in the Red Flour Beetle (Tribolium castaneum)" International Journal of Molecular Sciences 16, no. 4: 6631-6644. https://doi.org/10.3390/ijms16046631