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Int. J. Mol. Sci. 2015, 16(3), 6000-6017; doi:10.3390/ijms16036000

Locus Number Estimation of MHC Class II B in Stone Flounder and Japanese Flounder

Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China
These authors contributed equally to this work.
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Academic Editor: Ritva Tikkanen
Received: 2 December 2014 / Revised: 22 December 2014 / Accepted: 25 December 2014 / Published: 13 March 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Members of major histocompatibility complex (MHC) family are important in immune systems. Great efforts have been made to reveal their complicated gene structures. But many existing studies focus on partial sequences of MHC genes. In this study, by gene cloning and sequencing, we identified cDNA sequences and DNA sequences of the MHC class II B in two flatfishes, stone flounder (Kareius bicoloratus) and homozygous diploid Japanese flounder (Paralichthys olivaceus). Eleven cDNA sequences were acquired from eight stone flounder individuals, and most of the polymorphic sites distributed in exons 2 and 3. Twenty-eight alleles were identified from the DNA fragments in these eight individuals. It could be deduced from their Bayesian inference phylogenetic tree that at least four loci of MHC class II B exist in stone flounder. The detailed whole-length DNA sequences in one individual were analyzed, revealing that the intron length varied among different loci. Four different cDNA sequences were identified from one homozygous diploid Japanese flounder individual, implying the existence of at least four loci. Comparison of the cDNA sequences to the DNA sequence confirmed that six exons existed in this gene of Japanese flounder, which was a common feature shared by Pleuronectiformes fishes. Our results proved the multi-locus feature of MHC class II B. The sequences we obtained would provide detailed and systematic data for further research. View Full-Text
Keywords: major histocompatibility complex; MHC; class II B; stone flounder; Japanese flounder; locus number major histocompatibility complex; MHC; class II B; stone flounder; Japanese flounder; locus number
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Jiang, J.; Li, C.; Zhang, Q.; Wang, X. Locus Number Estimation of MHC Class II B in Stone Flounder and Japanese Flounder. Int. J. Mol. Sci. 2015, 16, 6000-6017.

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