Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain
AbstractWe recently isolated stem cells derived from the brain of a bovine fetus, utilizing a particular mechanical separation method. After improving our experimental conditions, we obtained neural stem cells using an optimized culture medium system. The cells were expanded, established in continuous cell culture and used for immunofluorescence cytochemistry. RT-PCR showed that embryonic neural stem cells (NSCs) not only expresses the protein Sox2, Nestin but also Pax6, Musashi proteins and were differentiated into the three classical neuronal phenotypes (neurons, astrocytes, and oligodendrocytes). View Full-Text
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Gao, Y.; Li, X.; Zheng, D.; Guan, W.; Ma, Y. Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain. Int. J. Mol. Sci. 2015, 16, 5990-5999.
Gao Y, Li X, Zheng D, Guan W, Ma Y. Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain. International Journal of Molecular Sciences. 2015; 16(3):5990-5999.Chicago/Turabian Style
Gao, Yuhua; Li, Xiangchen; Zheng, Dong; Guan, Weijun; Ma, Yuehui. 2015. "Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain." Int. J. Mol. Sci. 16, no. 3: 5990-5999.