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Int. J. Mol. Sci. 2015, 16(3), 5990-5999; doi:10.3390/ijms16035990

Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain

1,2,†
,
1,2,†
,
2
,
1,* and 1,*
1
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2
College of Wildlife Resources, Northeast Forestry University, Harbin 150040, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Xiaofeng Jia
Received: 22 December 2014 / Revised: 4 February 2015 / Accepted: 10 February 2015 / Published: 13 March 2015
(This article belongs to the Special Issue Neurological Injuries’ Monitoring, Tracking and Treatment)
View Full-Text   |   Download PDF [1480 KB, uploaded 13 March 2015]   |  

Abstract

We recently isolated stem cells derived from the brain of a bovine fetus, utilizing a particular mechanical separation method. After improving our experimental conditions, we obtained neural stem cells using an optimized culture medium system. The cells were expanded, established in continuous cell culture and used for immunofluorescence cytochemistry. RT-PCR showed that embryonic neural stem cells (NSCs) not only expresses the protein Sox2, Nestin but also Pax6, Musashi proteins and were differentiated into the three classical neuronal phenotypes (neurons, astrocytes, and oligodendrocytes). View Full-Text
Keywords: bovine; neural stem cell; differentiation bovine; neural stem cell; differentiation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Gao, Y.; Li, X.; Zheng, D.; Guan, W.; Ma, Y. Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain. Int. J. Mol. Sci. 2015, 16, 5990-5999.

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