A Perilipin Gene from Clonostachys rosea f. Catenulata HL-1-1 Is Related to Sclerotial Parasitism
AbstractClonostachys rosea f. catenulata is a promising biocontrol agent against many fungal plant pathogens. To identify mycoparasitism-related genes from C. rosea f. catenulata, a suppression subtractive hybridization (SSH) cDNA library of C. rosea f. catenulata HL-1-1 that parasitizes the sclerotia of S. sclerotiorum was constructed. 502 clones were sequenced randomly, and thereby 472 expressed sequence tags (ESTs) were identified. Forty-three unigenes were annotated and exhibited similarity to a wide diversity of genes. Quantitative real -time PCR showed that a perilipin-like protein encoding gene, Per3, was up-regulated by 6.6-fold over the control at 96 h under the induction of sclerotia. The full-length sequence of Per3 was obtained via 5' and 3' rapid identification of cDNA ends. Overexpression of Per3 in HL-1-1 significantly enhanced the parasitic ability on sclerotia. The results indicated that Per3 might be involved in the mycoparasitism of C. rosea f. catenulata HL-1-1. This is the first report of a perilipin as a potential biocontrol gene in mycoparasites. The study provides usefu l insights into the interaction between C. rosea f. catenulata and fungal plant pathogens. View Full-Text
- Supplementary File 1:
Supplementary Information (PDF, 1091 KB)
Share & Cite This Article
Sun, Z.-B.; Li, S.-D.; Zhong, Z.-M.; Sun, M.-H. A Perilipin Gene from Clonostachys rosea f. Catenulata HL-1-1 Is Related to Sclerotial Parasitism. Int. J. Mol. Sci. 2015, 16, 5347-5362.
Sun Z-B, Li S-D, Zhong Z-M, Sun M-H. A Perilipin Gene from Clonostachys rosea f. Catenulata HL-1-1 Is Related to Sclerotial Parasitism. International Journal of Molecular Sciences. 2015; 16(3):5347-5362.Chicago/Turabian Style
Sun, Zhan-Bin; Li, Shi-Dong; Zhong, Zeng-Ming; Sun, Man-Hong. 2015. "A Perilipin Gene from Clonostachys rosea f. Catenulata HL-1-1 Is Related to Sclerotial Parasitism." Int. J. Mol. Sci. 16, no. 3: 5347-5362.