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Int. J. Mol. Sci. 2015, 16(3), 5347-5362; doi:10.3390/ijms16035347

A Perilipin Gene from Clonostachys rosea f. Catenulata HL-1-1 Is Related to Sclerotial Parasitism

Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China
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Academic Editor: Bing Yan
Received: 9 January 2015 / Revised: 1 March 2015 / Accepted: 2 March 2015 / Published: 9 March 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Clonostachys rosea f. catenulata is a promising biocontrol agent against many fungal plant pathogens. To identify mycoparasitism-related genes from C. rosea f. catenulata, a suppression subtractive hybridization (SSH) cDNA library of C. rosea f. catenulata HL-1-1 that parasitizes the sclerotia of S. sclerotiorum was constructed. 502 clones were sequenced randomly, and thereby 472 expressed sequence tags (ESTs) were identified. Forty-three unigenes were annotated and exhibited similarity to a wide diversity of genes. Quantitative real -time PCR showed that a perilipin-like protein encoding gene, Per3, was up-regulated by 6.6-fold over the control at 96 h under the induction of sclerotia. The full-length sequence of Per3 was obtained via 5' and 3' rapid identification of cDNA ends. Overexpression of Per3 in HL-1-1 significantly enhanced the parasitic ability on sclerotia. The results indicated that Per3 might be involved in the mycoparasitism of C. rosea f. catenulata HL-1-1. This is the first report of a perilipin as a potential biocontrol gene in mycoparasites. The study provides usefu l insights into the interaction between C. rosea f. catenulata and fungal plant pathogens. View Full-Text
Keywords: clonostachys rosea f. catenulate; Sclerotinia sclerotiorum; sclerotia; perilipin; mycoparasitism; ESTs clonostachys rosea f. catenulate; Sclerotinia sclerotiorum; sclerotia; perilipin; mycoparasitism; ESTs
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Sun, Z.-B.; Li, S.-D.; Zhong, Z.-M.; Sun, M.-H. A Perilipin Gene from Clonostachys rosea f. Catenulata HL-1-1 Is Related to Sclerotial Parasitism. Int. J. Mol. Sci. 2015, 16, 5347-5362.

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