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Int. J. Mol. Sci. 2015, 16(3), 4997-5013; doi:10.3390/ijms16034997

Transcriptome MicroRNA Profiling of Bovine Mammary Glands Infected with Staphylococcus aureus

1
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
2
The Centre for the Analysis of Genome Evolution and Function (CAGEF), University of Toronto, Toronto, ON M5S 2J7, Canada
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Ritva Tikkanen
Received: 13 January 2015 / Revised: 19 February 2015 / Accepted: 23 February 2015 / Published: 4 March 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [2566 KB, uploaded 4 March 2015]   |  

Abstract

MicroRNAs are small non-coding RNA molecules that are important regulators of gene expression at the post-transcriptional level. miRNAs impact the processes of cell proliferation, differentiation and apoptosis. Thus, the regulation of miRNA expression profiles associated with mastitis will be conducive for its control. In this study, Staphylococcus aureus (S. aureus) was administered to the mammary gland of Chinese Holstein cows to construct a bacteria-type mastitis model. Total RNA was isolated from bovine mammary gland tissue samples from the S. aureus-induced mastitis group and controls. miRNAs were analyzed using Solexa sequencing and bioinformatics processing for the experimental group and control group. Two miRNA libraries were constructed respectively. A total of 370 known bovine miRNAs and 341 novel mi RNAs were detected for the S. aureus and 358 known bovine miRNAs and 232 novel miRNAs for control groups. A total of 77 miRNAs in the S. aureus group showed significant differences compared to the control group. GO (Gene Ontology) analysis showed these target genes were involved in the regulation of cells, binding, etc., while KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis showed that these genes were enriched in endocytosis, and olfactory transduction pathways involved in cancer. These results provide an experimental basis to reveal the cause and regulatory mechanism of mastitis and also suggest the potential of miRNAs to serve as biomarkers for the diagnosis of mastitis in dairy cows. View Full-Text
Keywords: microRNAs; mastitis; Staphylococcus aureus; Solexa sequencing; qRT-PCR microRNAs; mastitis; Staphylococcus aureus; Solexa sequencing; qRT-PCR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Li, R.; Zhang, C.-L.; Liao, X.-X.; Chen, D.; Wang, W.-Q.; Zhu, Y.-H.; Geng, X.-H.; Ji, D.-J.; Mao, Y.-J.; Gong, Y.-C.; Yang, Z.-P. Transcriptome MicroRNA Profiling of Bovine Mammary Glands Infected with Staphylococcus aureus. Int. J. Mol. Sci. 2015, 16, 4997-5013.

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