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Int. J. Mol. Sci. 2015, 16(2), 3990-3995; doi:10.3390/ijms16023990

Development of Biodegradable Nanocarriers Loaded with a Monoclonal Antibody

1
Department of Molecular and Medical Genetics, University of North Texas Health Science Center, 3500 Camp Bowie Blvd, Fort Worth, TX 76107, USA
2
Texas College of Osteopathic Medicine, University of North Texas Health Science Center, 3500 Camp Bowie Blvd, Fort Worth, TX 76107, USA
3
Institute of Cancer Research, University of North Texas Health Science Center, 3500 Camp Bowie Blvd, Fort Worth, TX 76107, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Bing Yan
Received: 23 December 2014 / Revised: 21 January 2015 / Accepted: 28 January 2015 / Published: 12 February 2015
(This article belongs to the Special Issue Bioactive Nanoparticles 2014)
View Full-Text   |   Download PDF [983 KB, uploaded 12 February 2015]   |  

Abstract

Treatments utilizing monoclonal antibody therapeutics against intracellular protein-protein interactions in cancer cells have been hampered by several factors, including poor intracellular uptake and rapid lysosomal degradation. Our current work examines the feasibility of encapsulating monoclonal antibodies within poly(lactic-co-glycolic acid) (PLGA) nanoparticles using a water/oil/water double emulsion solvent evaporation technique. This method can be used to prepare protective polymeric nanoparticles for transporting functional antibodies to the cytoplasmic compartment of cancer cells. Nanoparticles were formulated and then characterized using a number of physical and biological parameters. The average nanoparticle size ranged from 221 to 252 nm with a low polydispersity index. Encapsulation efficiency of 16%–22% and antibody loading of 0.3%–1.12% were observed. The antibody molecules were released from the nanoparticles in a sustained manner and upon release maintained functionality. Our studies achieved successful formulation of antibody loaded polymeric nanoparticles, thus indicating that a PLGA-based antibody nanoformulation is a promising intracellular delivery vehicle for a large number of new intracellular antibody targets in cancer cells. View Full-Text
Keywords: antibody; nanoparticle; cancer; PLGA; therapy; formulation antibody; nanoparticle; cancer; PLGA; therapy; formulation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Gdowski, A.; Ranjan, A.; Mukerjee, A.; Vishwanatha, J. Development of Biodegradable Nanocarriers Loaded with a Monoclonal Antibody. Int. J. Mol. Sci. 2015, 16, 3990-3995.

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