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Int. J. Mol. Sci. 2015, 16(2), 3996-4006; doi:10.3390/ijms16023996

Effect of Porcine Akirin2 on Skeletal Myosin Heavy Chain Isoform Expression

1
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
2
College of Science, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
*
Author to whom correspondence should be addressed.
Academic Editor: Julian Borejdo
Received: 7 December 2014 / Revised: 4 February 2015 / Accepted: 9 February 2015 / Published: 12 February 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [2019 KB, uploaded 12 February 2015]   |  

Abstract

Akirin2 plays an important role in skeletal myogenesis. In this study, we found that porcine Akirin2 (pAkirin2) mRNA level was significantly higher in fast extensor digitorum longus (EDL) and longissimus lumborum (LL) muscles than in slow soleus (SOL) muscle of pigs. Overexpression of pAkirin2 increased the number of myosin heavy chain (MHC)-positive cells, indicating that pAkirin2 promoted myoblast differentiation. We also found that overexpression of pAkirin2 increased the mRNA expressions of MHCI and MHCIIa and decreased the mRNA expression of MHCIIb. Myocyte enhancer factor 2 (MEF2) and nuclear factor of activated T cells (NFAT) are the major downstream effectors of calcineurin. Here we also observed that the mRNA expressions of MEF2C and NFATc1 were notably elevated by pAkirin2 overexpression. Together, our data indicate that the role of pAkirin2 in modulating MHCI and MHCIIa expressions may be achieved through calcineurin/NFATc1 signaling pathway. View Full-Text
Keywords: porcine Akirin2; myosin heavy chain; myoblast differentiation; myocyte enhancer factor 2; nuclear factor of activated T cells porcine Akirin2; myosin heavy chain; myoblast differentiation; myocyte enhancer factor 2; nuclear factor of activated T cells
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Chen, X.; Luo, Y.; Zhou, B.; Huang, Z.; Jia, G.; Liu, G.; Zhao, H.; Yang, Z.; Zhang, R. Effect of Porcine Akirin2 on Skeletal Myosin Heavy Chain Isoform Expression. Int. J. Mol. Sci. 2015, 16, 3996-4006.

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