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Int. J. Mol. Sci. 2015, 16(2), 3178-3187; doi:10.3390/ijms16023178

CXCL13 Promotes the Effect of Bone Marrow Mesenchymal Stem Cells (MSCs) on Tendon-Bone Healing in Rats and in C3HIOT1/2 Cells

Department of Orthopaedic Surgery, Shengjing Hospital of China Medical University, Shenyang 110024, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: William Chi-shing Cho
Received: 13 November 2014 / Revised: 14 January 2015 / Accepted: 21 January 2015 / Published: 30 January 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Objectives: Mesenchymal stem cells (MSCs) are potential effective therapy for tissue repair and bone regeneration. In present study, the effects of CXC chemokine ligand-13 (CXCL13) were evaluated on tendon-bone healing of rats. Methods: Tendon bone healing of the rat model was established and biomechanical testing was performed at 2, 4, 8 weeks after surgery. Murine mesenchymal cell line (C3HIOT1/2 cells) was cultured. The expression of miRNA-23a was detected by real-time PCR. The protein expression of ERK1/2, JNK and p38 was detected by western blotting. MiR-23a mimic and inhibitor were used to overexpress or silence the expression of miR-23a. Results: MSCs significantly elevated the levels of ultimate load to failure, stiffness and stress in specimens of rats, the effects of which were enhanced by CXCL13. The expression of miR-23a was down-regulated and the protein of ERK1/2 level was up-regulated by CXCL13 treatment in both in vivo and in vitro experiments. ERK1/2 expression was elevated by overexpression of miR-23a and reduced by miR-23a inhibitor. Conclusions: These findings revealed that CXCL13 promoted the tendon-bone healing in rats with MSCs treatment, and implied that the activation of ERK1/2 via miR-23a was involved in the process of MSCs treated bone regeneration. View Full-Text
Keywords: marrow mesenchymal stem cells; rat; tendon-bone healing; CXCL13 marrow mesenchymal stem cells; rat; tendon-bone healing; CXCL13
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Tian, F.; Ji, X.-L.; Xiao, W.-A.; Wang, B.; Wang, F. CXCL13 Promotes the Effect of Bone Marrow Mesenchymal Stem Cells (MSCs) on Tendon-Bone Healing in Rats and in C3HIOT1/2 Cells. Int. J. Mol. Sci. 2015, 16, 3178-3187.

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