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Int. J. Mol. Sci. 2015, 16(2), 3116-3132; doi:10.3390/ijms16023116

Optimization of Influential Nutrients during Direct Cellulose Fermentation into Hydrogen by Clostridium thermocellum

1
Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
2
Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
*
Author to whom correspondence should be addressed.
Academic Editor: Patrick Hallenbeck
Received: 4 December 2014 / Revised: 22 January 2015 / Accepted: 27 January 2015 / Published: 30 January 2015
(This article belongs to the Special Issue Photosynthesis and Biological Hydrogen Production)
View Full-Text   |   Download PDF [813 KB, uploaded 2 February 2015]   |  

Abstract

Combinatorial effects of influential growth nutrients were investigated in order to enhance hydrogen (H2) production during direct conversion of cellulose by Clostridium thermocellum DSM 1237. A central composite face-centered design and response surface methodology (RSM) were applied to optimize concentrations of cellulose, yeast extract (YE), and magnesium chloride (Mg) in culture. The overall optimum composition generated by the desirability function resulted in 57.28 mmol H2/L-culture with 1.30 mol H2/mol glucose and 7.48 mmol/(g·cell·h) when cultures contained 25 g/L cellulose, 2 g/L YE, and 1.75 g/L Mg. Compared with the unaltered medium, the optimized medium produced approximately 3.2-fold more H2 within the same time-frame with 50% higher specific productivity, which are also better than previously reported values from similar studies. Nutrient composition that diverted carbon and electron flux away from H2 promoting ethanol production was also determined. This study represents the first investigation dealing with multifactor optimization with RSM for H2 production during direct cellulose fermentation. View Full-Text
Keywords: cellulose; Clostridum thermocellum; medium composition; optimization; central composite design cellulose; Clostridum thermocellum; medium composition; optimization; central composite design
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Islam, R.; Sparling, R.; Cicek, N.; Levin, D.B. Optimization of Influential Nutrients during Direct Cellulose Fermentation into Hydrogen by Clostridium thermocellum. Int. J. Mol. Sci. 2015, 16, 3116-3132.

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