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Int. J. Mol. Sci. 2015, 16(2), 3035-3057; doi:10.3390/ijms16023035

Transcriptome Analysis of Methyl Jasmonate-Elicited Panax ginseng Adventitious Roots to Discover Putative Ginsenoside Biosynthesis and Transport Genes

Molecular Biology Research Center, State Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha 410078, China
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Academic Editor: Marcello Iriti
Received: 12 December 2014 / Accepted: 22 January 2015 / Published: 29 January 2015
(This article belongs to the Special Issue Plant Molecular Biology)
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Abstract

The Panax ginseng C.A. Meyer belonging to the Araliaceae has long been used as an herbal medicine. Although public databases are presently available for this family, no methyl jasmonate (MeJA) elicited transcriptomic information was previously reported on this species, with the exception of a few expressed sequence tags (ESTs) using the traditional Sanger method. Here, approximately 53 million clean reads of adventitious root transcriptome were separately filtered via Illumina HiSeq™2000 from two samples treated with MeJA (Pg-MeJA) and equal volumes of solvent, ethanol (Pg-Con). Jointly, a total of 71,095 all-unigenes from both samples were assembled and annotated, and based on sequence similarity search with known proteins, a total of 56,668 unigenes was obtained. Out of these annotated unigenes, 54,920 were assigned to the NCBI non-redundant protein (Nr) database, 35,448 to the Swiss-prot database, 43,051 to gene ontology (GO), and 19,986 to clusters of orthologous groups (COG). Searching in the Kyoto encyclopedia of genes and genomes (KEGG) pathway database indicated that 32,200 unigenes were mapped to 128 KEGG pathways. Moreover, we obtained several genes showing a wide range of expression levels. We also identified a total of 749 ginsenoside biosynthetic enzyme genes and 12 promising pleiotropic drug resistance (PDR) genes related to ginsenoside transport. View Full-Text
Keywords: Panax ginseng; adventitious root; methyl jasmonate; Illumina/Solexa; transcriptome; ginsenoside biosynthesis; PDR (pleiotropic drug resistance) transporters; MVA (mevalonic acid) pathway Panax ginseng; adventitious root; methyl jasmonate; Illumina/Solexa; transcriptome; ginsenoside biosynthesis; PDR (pleiotropic drug resistance) transporters; MVA (mevalonic acid) pathway
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Cao, H.; Nuruzzaman, M.; Xiu, H.; Huang, J.; Wu, K.; Chen, X.; Li, J.; Wang, L.; Jeong, J.-H.; Park, S.-J.; Yang, F.; Luo, J.; Luo, Z. Transcriptome Analysis of Methyl Jasmonate-Elicited Panax ginseng Adventitious Roots to Discover Putative Ginsenoside Biosynthesis and Transport Genes. Int. J. Mol. Sci. 2015, 16, 3035-3057.

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