Next Article in Journal
Pacemaker Activity of the Human Sinoatrial Node: An Update on the Effects of Mutations in HCN4 on the Hyperpolarization-Activated Current
Next Article in Special Issue
Genome-Wide Identification and Analysis of Drought-Responsive Genes and MicroRNAs in Tobacco
Previous Article in Journal
Transcriptome Analysis of Methyl Jasmonate-Elicited Panax ginseng Adventitious Roots to Discover Putative Ginsenoside Biosynthesis and Transport Genes
Previous Article in Special Issue
Reactive Oxygen and Nitrogen Species in Defense/Stress Responses Activated by Chitosan in Sycamore Cultured Cells
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2015, 16(2), 3058-3070; doi:10.3390/ijms16023058

A Ribosomal Protein AgRPS3aE from Halophilic Aspergillus glaucus Confers Salt Tolerance in Heterologous Organisms

1
College of Plant Sciences, Jilin University, Changchun 130062, China
2
Agricultural College, Heilongjiang Bayi Agricultural University, Daqing 163319, China
These authors are equally contributed to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Ann Cuypers
Received: 17 December 2014 / Revised: 30 December 2014 / Accepted: 21 January 2015 / Published: 29 January 2015
(This article belongs to the Special Issue Abiotic Stress and Gene Networks in Plants)
View Full-Text   |   Download PDF [2634 KB, uploaded 29 January 2015]   |  

Abstract

High salt in soils is one of the abiotic stresses that significantly reduces crop yield, although saline lands are considered potential resources arable for agriculture. Currently, genetic engineering for enhancing salt tolerance is being tested as an efficient and viable strategy for crop improvement. We previously characterized a large subunit of the ribosomal protein RPL44, which is involved in osmotic stress in the extremely halophilic fungus Aspergillus glaucus. Here, we screened another ribosomal protein (AgRPS3aE) that also produced high-salt tolerance in yeast. Bioinformatics analysis indicated that AgRPS3aE encodes a 29.2 kDa small subunit of a ribosomal protein belonging to the RPS3Ae family in eukaryotes. To further confirm its protective function against salinity, we expressed AgRPS3aE in three heterologous systems, the filamentous fungus Magnaporthe oryzae and two model plants Arabidopsis and tobacco. Overexpression of AgRPS3aE in all tested transformants significantly alleviated stress symptoms compared with controls, suggesting that AgRPS3aE functions not only in fungi but also in plants. Considering that ribosomal proteins are housekeeping components in organisms from prokaryotes to eukaryotes, we propose that AgRPS3aE is one of the optimal genes for improving high-salt tolerance in crops. View Full-Text
Keywords: Aspergillus glaucus; ribosomal protein AgRPS3aE; salt tolerance; expression in hetero-organisms Aspergillus glaucus; ribosomal protein AgRPS3aE; salt tolerance; expression in hetero-organisms
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Liang, X.; Liu, Y.; Xie, L.; Liu, X.; Wei, Y.; Zhou, X.; Zhang, S. A Ribosomal Protein AgRPS3aE from Halophilic Aspergillus glaucus Confers Salt Tolerance in Heterologous Organisms. Int. J. Mol. Sci. 2015, 16, 3058-3070.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top