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Int. J. Mol. Sci. 2015, 16(2), 2663-2677; doi:10.3390/ijms16022663

Bone-Remodeling Transcript Levels Are Independent of Perching in End-of-Lay White Leghorn Chickens

1
School of Biological Sciences, Illinois State University, Normal, IL 61701, USA
2
Department of Biology, University of South Alabama, Mobile, AL 36688, USA
3
Department of Animal Sciences, Purdue University, 125 South Russell St, West Lafayette, IN 47907, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Ritva Tikkanen
Received: 26 November 2014 / Revised: 12 January 2015 / Accepted: 16 January 2015 / Published: 23 January 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [727 KB, uploaded 23 January 2015]   |  

Abstract

Osteoporosis is a bone disease that commonly results in a 30% incidence of fracture in hens used to produce eggs for human consumption. One of the causes of osteoporosis is the lack of mechanical strain placed on weight-bearing bones. In conventionally-caged hens, there is inadequate space for chickens to exercise and induce mechanical strain on their bones. One approach is to encourage mechanical stress on bones by the addition of perches to conventional cages. Our study focuses on the molecular mechanism of bone remodeling in end-of-lay hens (71 weeks) with access to perches. We examined bone-specific transcripts that are actively involved during development and remodeling. Using real-time quantitative PCR, we examined seven transcripts (COL2A1 (collagen, type II, alpha 1), RANKL (receptor activator of nuclear factor kappa-B ligand), OPG (osteoprotegerin), PTHLH (PTH-like hormone), PTH1R (PTH/PTHLH type-1 receptor), PTH3R (PTH/PTHLH type-3 receptor), and SOX9 (Sry-related high mobility group box)) in phalange, tibia and femur. Our results indicate that the only significant effect was a difference among bones for COL2A1 (femur > phalange). Therefore, we conclude that access to a perch did not alter transcript expression. Furthermore, because hens have been used as a model for human bone metabolism and osteoporosis, the results indicate that bone remodeling due to mechanical loading in chickens may be a product of different pathways than those involved in the mammalian model. View Full-Text
Keywords: laying hen; qPCR; bone-specific transcripts; bone-remodeling; signaling network laying hen; qPCR; bone-specific transcripts; bone-remodeling; signaling network
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Dale, M.D.; Mortimer, E.M.; Kolli, S.; Achramowicz, E.; Borchert, G.; Juliano, S.A.; Halkyard, S.; Sietz, N.; Gatto, C.; Hester, P.Y.; Rubin, D.A. Bone-Remodeling Transcript Levels Are Independent of Perching in End-of-Lay White Leghorn Chickens. Int. J. Mol. Sci. 2015, 16, 2663-2677.

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