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Int. J. Mol. Sci. 2015, 16(12), 30061-30074; doi:10.3390/ijms161226200

Biochemical Characterization of An Arginine-Specific Alkaline Trypsin from Bacillus licheniformis

School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Academic Editor: Ritva Tikkanen
Received: 8 November 2015 / Revised: 8 December 2015 / Accepted: 9 December 2015 / Published: 17 December 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

In the present study, we isolated a trypsin-producing strain DMN6 from the leather waste and identified it as Bacillus licheniformis through a two-step screening strategy. The trypsin activity was increased up to 140 from 20 U/mL through culture optimization. The enzyme was purified to electrophoretic homogeneity with a molecular mass of 44 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the specific activity of purified enzyme is 350 U/mg with Nα-Benzoyl-l-arginine ethylester as the substrate. The optimum temperature and pH for the trypsin are 65 °C and pH 9.0, respectively. Also, the enzyme can be significantly activated by Ba2+. This enzyme is relatively stable in alkaline environment and displays excellent activity at low temperatures. It could retain over 95% of enzyme activity after 180 min of incubation at 45 °C. The distinguished activity under low temperature and prominent stability enhance its catalytic potential. In the current work, the open reading frame was obtained with a length of 1371 nucleotides that encoded a protein of 456 amino acids. These data would warrant the B. licheniformis trypsin as a promising candidate for catalytic application in collagen preparation and leather bating through further protein engineering. View Full-Text
Keywords: trypsin; Bacillus licheniformis; enzymatic properties; cloning; biocatalysis trypsin; Bacillus licheniformis; enzymatic properties; cloning; biocatalysis
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MDPI and ACS Style

Gong, J.-S.; Li, W.; Zhang, D.-D.; Xie, M.-F.; Yang, B.; Zhang, R.-X.; Li, H.; Lu, Z.-M.; Xu, Z.-H.; Shi, J.-S. Biochemical Characterization of An Arginine-Specific Alkaline Trypsin from Bacillus licheniformis. Int. J. Mol. Sci. 2015, 16, 30061-30074.

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