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Int. J. Mol. Sci. 2015, 16(11), 26166-26185; doi:10.3390/ijms161125945

Homeodomain Protein Scr Regulates the Transcription of Genes Involved in Juvenile Hormone Biosynthesis in the Silkworm

1
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China
2
The Sericultural Research Institute, Jiangsu University of Science and Technology, Jiangsu 212018, China
3
The Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Jiangsu 212018, China
4
Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Lee A. Bulla
Received: 12 September 2015 / Revised: 13 October 2015 / Accepted: 23 October 2015 / Published: 2 November 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

The silkworm Dominant trimolting (Moltinism, M3) mutant undergoes three larval molts and exhibits precocious metamorphosis. In this study, we found that compared with the wild-type (WT) that undergoes four larval molts, both the juvenile hormone (JH) concentration and the expression of the JH-responsive gene Krüppel homolog 1 (Kr-h1) began to be greater in the second instar of the M3 mutant. A positional cloning analysis revealed that only the homeodomain transcription factor gene Sex combs reduced (Scr) is located in the genomic region that is tightly linked to the M3 locus. The expression level of the Scr gene in the brain-corpora cardiaca-corpora allata (Br-CC-CA) complex, which controls the synthesis of JH, was very low in the final larval instar of both the M3 and WT larvae, and exhibited a positive correlation with JH titer changes. Importantly, luciferase reporter analysis and electrophoretic mobility shift assay (EMSA) demonstrated that the Scr protein could promote the transcription of genes involved in JH biosynthesis by directly binding to the cis-regulatory elements (CREs) of homeodomain protein on their promoters. These results conclude that the homeodomain protein Scr is transcriptionally involved in the regulation of JH biosynthesis in the silkworm. View Full-Text
Keywords: silkworm; sex combs reduced; juvenile hormone; biosynthesis; transcriptional regulation silkworm; sex combs reduced; juvenile hormone; biosynthesis; transcriptional regulation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Meng, M.; Liu, C.; Peng, J.; Qian, W.; Qian, H.; Tian, L.; Li, J.; Dai, D.; Xu, A.; Li, S.; Xia, Q.; Cheng, D. Homeodomain Protein Scr Regulates the Transcription of Genes Involved in Juvenile Hormone Biosynthesis in the Silkworm. Int. J. Mol. Sci. 2015, 16, 26166-26185.

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