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Int. J. Mol. Sci. 2015, 16(10), 25691-25710; doi:10.3390/ijms161025691

ClRTL1 Encodes a Chinese Fir RNase III–Like Protein Involved in Regulating Shoot Branching

1
Key Laboratory of Forest Genetics and Biotechnology of the Ministry of Education of China, Nanjing Forestry University, Nanjing 210037, China
2
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China
3
Southern Mountain Timber Forest Cultivation Lab, Fujian Academy of Forestry, Ministry of Forestry, Fuzhou 350012, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Received: 27 May 2015 / Revised: 30 September 2015 / Accepted: 30 September 2015 / Published: 26 October 2015
(This article belongs to the Special Issue Molecular Research in Plant Secondary Metabolism 2015)
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Abstract

Identification of genes controlling shoot branching is crucial for improving plant architecture and increasing crop yield or biomass. A branching mutant of Chinese fir named “Dugansha” (Cunninghamia lanceolata var. dugan.) has been isolated in our laboratory. We chose the cDNA-AFLP technique and an effective strategy to screen genes that potentially regulate shoot branching in Chinese fir using this mutant. An RNase III-like1 cDNA fragment named ClRTL1 was identified as a potential positive regulator. To investigate the function of ClRTL1 in regulating shoot branching, we cloned the full-length cDNA sequence from C. lanceolata (Lamb.) Hook, deduced its secondary structure and function, and overexpressed the coding sequence in Arabidopsis. The ClRTL1 cDNA is 1045 bp and comprises an open reading frame of 705 bp. It encodes a protein of 235 amino acids. The deduced secondary structure of the ClRTL1 indicates that it is a mini-RNase III-like protein. The expression analysis and phenotypes of 35S: ClRTL1 in A. thaliana implies that ClRTL1 plays a role in promoting shoot branching in Chinese fir. View Full-Text
Keywords: Cunninghamia lanceolata (Lamb) Hook; branching; cDNA amplified fragment length polymorphism analysis; real time-PCR; overexpression; axillary bud Cunninghamia lanceolata (Lamb) Hook; branching; cDNA amplified fragment length polymorphism analysis; real time-PCR; overexpression; axillary bud
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Li, X.; Su, Q.; Zheng, R.; Liu, G.; Lu, Y.; Bian, L.; Chen, J.; Shi, J. ClRTL1 Encodes a Chinese Fir RNase III–Like Protein Involved in Regulating Shoot Branching. Int. J. Mol. Sci. 2015, 16, 25691-25710.

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