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Int. J. Mol. Sci. 2015, 16(10), 25678-25690; doi:10.3390/ijms161025678

The Effect of Interferon-γ and Zoledronate Treatment on Alpha-Tricalcium Phosphate/Collagen Sponge-Mediated Bone-Tissue Engineering

1
Department of Orthodontics, Graduate School of Dentistry, Osaka Dental University, 8-1 Kuzuha Hanazonocho, Hirakata, Osaka 573-1121, Japan
2
Department of Biomaterials, Osaka Dental University, 8-1 Kuzuha Hanazonocho, Hirakata, Osaka 573-1121, Japan
3
Institute of Dental Research, Osaka Dental University, 8-1 Kuzuha Hanazonocho, Hirakata, Osaka 573-1121, Japan
*
Authors to whom correspondence should be addressed.
Academic Editor: Mohamed Rahaman
Received: 14 July 2015 / Revised: 15 October 2015 / Accepted: 15 October 2015 / Published: 26 October 2015
(This article belongs to the Special Issue Biomaterials for Tissue Engineering)
View Full-Text   |   Download PDF [2151 KB, uploaded 26 October 2015]   |  

Abstract

Inflammatory responses are frequently associated with the expression of inflammatory cytokines and severe osteoclastogenesis, which significantly affect the efficacy of biomaterials. Recent findings have suggested that interferon (IFN)-γ and zoledronate (Zol) are effective inhibitors of osteoclastogenesis. However, little is known regarding the utility of IFN-γ and Zol in bone tissue engineering. In this study, we generated rat models by generating critically sized defects in calvarias implanted with an alpha-tricalcium phosphate/collagen sponge (α-TCP/CS). At four weeks post-implantation, the rats were divided into IFN-γ, Zol, and control (no treatment) groups. Compared with the control group, the IFN-γ and Zol groups showed remarkable attenuation of severe osteoclastogenesis, leading to a significant enhancement in bone mass. Histomorphometric data and mRNA expression patterns in IFN-γ and Zol-injected rats reflected high bone-turnover with increased bone formation, a reduction in osteoclast numbers, and tumor necrosis factor-α expression. Our results demonstrated that the administration of IFN-γ and Zol enhanced bone regeneration of α-TCP/CS implants by enhancing bone formation, while hampering excess bone resorption. View Full-Text
Keywords: bone tissue engineering; α-TCP/CS; inflammatory response; RANKL; TNF-α; osteoclastogenesis; interferon-γ; zoledronate bone tissue engineering; α-TCP/CS; inflammatory response; RANKL; TNF-α; osteoclastogenesis; interferon-γ; zoledronate
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Li, P.; Hashimoto, Y.; Honda, Y.; Arima, Y.; Matsumoto, N. The Effect of Interferon-γ and Zoledronate Treatment on Alpha-Tricalcium Phosphate/Collagen Sponge-Mediated Bone-Tissue Engineering. Int. J. Mol. Sci. 2015, 16, 25678-25690.

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