Genome Editing Using Mammalian Haploid Cells
AbstractHaploid cells are useful for studying gene functions because disruption of a single allele can cause loss-of-function phenotypes. Recent success in generating haploid embryonic stem cells (ESCs) in mice, rats, and monkeys provides a new platform for simple genetic manipulation of the mammalian genome. Use of haploid ESCs enhances the genome-editing potential of the CRISPR/Cas system. For example, CRISPR/Cas was used in haploid ESCs to generate multiple knockouts and large deletions at high efficiency. In addition, genome-wide screening is facilitated by haploid cell lines containing gene knockout libraries. View Full-Text
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Horii, T.; Hatada, I. Genome Editing Using Mammalian Haploid Cells. Int. J. Mol. Sci. 2015, 16, 23604-23614.
Horii T, Hatada I. Genome Editing Using Mammalian Haploid Cells. International Journal of Molecular Sciences. 2015; 16(10):23604-23614.Chicago/Turabian Style
Horii, Takuro; Hatada, Izuho. 2015. "Genome Editing Using Mammalian Haploid Cells." Int. J. Mol. Sci. 16, no. 10: 23604-23614.