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Int. J. Mol. Sci. 2015, 16(9), 22151-22168; doi:10.3390/ijms160922151

CRISPR-Cas9: A Revolutionary Tool for Cancer Modelling

1
Viral Vector Technical Unit, Fundacion Centro Nacional de Investigaciones Cardiovasculares (CNIC), Melchor Fernandez Almagro, 3, 28029 Madrid, Spain
2
Molecular Cytogenetics Group, Human Cancer Genetics Program, Centro Nacional de Investigaciones Oncológicas (CNIO), Melchor Fernandez Almagro, 3, 28029 Madrid, Spain
*
Authors to whom correspondence should be addressed.
Academic Editor: Izuho Hatada
Received: 22 August 2015 / Revised: 3 September 2015 / Accepted: 6 September 2015 / Published: 14 September 2015
(This article belongs to the Special Issue Genome Editing)
View Full-Text   |   Download PDF [856 KB, uploaded 14 September 2015]   |  

Abstract

The cancer-modelling field is now experiencing a conversion with the recent emergence of the RNA-programmable CRISPR-Cas9 system, a flexible methodology to produce essentially any desired modification in the genome. Cancer is a multistep process that involves many genetic mutations and other genome rearrangements. Despite their importance, it is difficult to recapitulate the degree of genetic complexity found in patient tumors. The CRISPR-Cas9 system for genome editing has been proven as a robust technology that makes it possible to generate cellular and animal models that recapitulate those cooperative alterations rapidly and at low cost. In this review, we will discuss the innovative applications of the CRISPR-Cas9 system to generate new models, providing a new way to interrogate the development and progression of cancers. View Full-Text
Keywords: CRISPR-Cas9 system; cancer modelling; gene mutations; rearrangements; genome engineering CRISPR-Cas9 system; cancer modelling; gene mutations; rearrangements; genome engineering
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Torres-Ruiz, R.; Rodriguez-Perales, S. CRISPR-Cas9: A Revolutionary Tool for Cancer Modelling. Int. J. Mol. Sci. 2015, 16, 22151-22168.

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