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Int. J. Mol. Sci. 2015, 16(10), 23143-23164; doi:10.3390/ijms161023143

Applications of Engineered DNA-Binding Molecules Such as TAL Proteins and the CRISPR/Cas System in Biology Research

Chromatin Biochemistry Research Group, Combined Program on Microbiology and Immunology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan
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Author to whom correspondence should be addressed.
Academic Editor: Qiang “Shawn” Chen
Received: 10 August 2015 / Revised: 31 August 2015 / Accepted: 14 September 2015 / Published: 24 September 2015
(This article belongs to the Special Issue Protein Engineering)
View Full-Text   |   Download PDF [1517 KB, uploaded 24 September 2015]   |  

Abstract

Engineered DNA-binding molecules such as transcription activator-like effector (TAL or TALE) proteins and the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) (CRISPR/Cas) system have been used extensively for genome editing in cells of various types and species. The sequence-specific DNA-binding activities of these engineered DNA-binding molecules can also be utilized for other purposes, such as transcriptional activation, transcriptional repression, chromatin modification, visualization of genomic regions, and isolation of chromatin in a locus-specific manner. In this review, we describe applications of these engineered DNA-binding molecules for biological purposes other than genome editing. View Full-Text
Keywords: TAL; TALE; CRISPR/Cas; Cas9; dCas9; sgRNA; transcription; live imaging; locus-specific ChIP; enChIP TAL; TALE; CRISPR/Cas; Cas9; dCas9; sgRNA; transcription; live imaging; locus-specific ChIP; enChIP
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Fujita, T.; Fujii, H. Applications of Engineered DNA-Binding Molecules Such as TAL Proteins and the CRISPR/Cas System in Biology Research. Int. J. Mol. Sci. 2015, 16, 23143-23164.

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